Oxidative stress and inflammatory response biomarkers in dogs with mammary carcinoma

Author(s):Show less -

Machado, Vanessa S. ^[1] ; Crivellenti, Leandro Z. ^{[2, 3]} ; Bottari, Nathieli B. ^[4] ; Tonin, Alexandre A. ^[5] ; Pelinson, Luana P. ^[4] ; Borin-Crivellenti, Sofia ^[2] ; Santana, Aureo E. ^[2] ; Torbitz, Vanessa D. ^[6] ; Moresco, Rafael N. ^[6] ; Duarte, Thiago ^[7] ; Duarte, Marta M. M. F. ^[8] ; Schetinger, Maria Rosa C. ^[4] ; Morschd, Vera M. ^[4] ; Jaques, Jeandre A. ^[9] ; Tinucci-Costa, Mirela ^[2] ; Da Silva, Aleksandro S. ^{[1, 10]}

Affiliation

^[1] Univ Fed Santa Maria, Dept Microbiol & Parasitol, Santa Maria, RS - Brazil

^[2] Sao Paulo State Univ UNESP, Dept Vet Clin & Surg, Jaboticabal - Brazil

^[3] Franca Univ UNIFRAN, Dept Vet Clin & Surg, Franca - Brazil

^[4] Univ Fed Santa Maria, Dept Biochem & Mol Biol, Santa Maria, RS - Brazil

^[5] Univ Oeste Santa Catarina UNOESC, Xanxere - Brazil

^[6] Univ Fed Santa Maria, Dept Clin & Toxicol Anal, BR-97119900 Santa Maria, RS - Brazil

^[7] Univ Fed Santa Maria, Dept Farmacol, Santa Maria, RS - Brazil

^[8] Univ Luterana Brasil, Santa Maria, RS - Brazil

^[9] Univ Fed Mato Grosso Sul UFMS, Ctr Ciencias Biol & Saude, Lab Gen Biochem & Microorganisms, Campo Grande, MS - Brazil

^[10] Univ Estado Santa Catarina UDESC, Dept Anim Sci, Chapeco, SC - Brazil

• Document type: Journal article
• Source: PATHOLOGY RESEARCH AND PRACTICE; v. 211, n. 9, p. 677-681, 2015.
• Web of Science Citations: 6

Abstract

Mammary carcinoma is the most common cancer that affects dogs, and in many cases it leads to death. Thus, given the importance of this disease, to clarify its pathogenesis is an important measure. In this sense, the aim of this study was to investigate the levels of cytokines and nitric oxide (NO), oxidative and antioxidant status, as well as the activity of adenosine deaminase (ADA) and butyrylcholinesterase (BChE) in dogs diagnosed with mammary carcinoma. With this purpose, thirty-three (33) serum samples from female dogs with histopathological diagnosis of mammary carcinoma, without evidence of metastasis, were used (group B). The material was classified based on the degree of malignancy, as follows: subgroup B1 (low-grade malignancy; n=26) and subgroup B2 (high grade of malignancy; n=7). Serum samples from healthy females (group A; n=10) were used as negative control. Our results showed that levels of cytokines (TNF-alpha, INF-gamma, IL-1, and IL-6), NO (nitrite/nitrate), AOPP (protein oxidation), and FRAP (antioxidant power) were significantly (P<0.05) increased in dogs with mammary carcinoma (group B), when compared with group A. On the other hand, ADA activity was significantly decreased (P<0.05) in both subgroups B1 and B2, when compared with group A. BChE activity, however, was reduced (P<0.05) only in subgroup B2 when compared with group A and subgroup B1. Unlike other variables, NO, AOPP, and IFN-gamma were influenced by the degree of tumor malignancy, i.e., their levels were even higher in subgroup B2. Therefore, based on these results, we can conclude that all variables investigated are related to the pathogenesis of this disease, since they were altered in dogs with mammary tumor. Additionally, we suggest that ADA activity had an anti-inflammatory effect on these tumor samples, probably in order to modulate the inflammatory response. (C) 2015 Elsevier GmbH. All rights reserved. (AU)

• FAPESP's process: 11/11355-9 - Evaluation of glomerular tubulointerstitial changes in bitches with mammary neoplasia
• Grantee: Áureo Evangelista Santana
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/08416-6 - Correlation between tubule-clusters alterations and the degree of histology mammary neoplasm in bitches
• Grantee: Leandro Zuccolotto Crivellenti
• Support Opportunities: Scholarships in Brazil - Doctorate