Treatment of periodontal disease with an Er,Cr:YSGG laser in rats exposed to cigarette smoke inhalation

Author(s)

Pavone, Chaine ^[1] ; Boas Nogueira, Andressa Vilas ^[1] ; Pimentel Lopes de Oliveira, Guilherme Jose ^[1] ; Scardueli, Cassio Rocha ^[1] ; Cerri, Paulo Sergio ^[1] ; Marcantonio, Jr., Elcio ^[1] ; Chierici Marcantonio, Rosemary Adriana ^[1]

Affiliation

^[1] Sao Paulo Univ Julio de Mesquita Filho, Araraquara Dent Sch, Araraquara, SP - Brazil

• Document type: Journal article
• Source: Lasers in Medical Science; v. 30, n. 8, p. 2095-2103, NOV 2015.
• Web of Science Citations: 3

Abstract

The purpose of this study was to evaluate the erbium, chromium:yttrium-scandiumgallium-garnet (Er,Cr:YSGG) laser irradiation in the treatment of periodontitis in rats exposed to cigarette smoke inhalation (CSI). Ligatures were placed in the maxillary second molars. After a 15-day period, the ligatures were removed and 180 animals were randomly divided into six groups: (1) CSRP group-CSI and manual scaling and root planing (SRP) treatment; (2) CL group-CSI and Er,Cr:YSGG laser irradiation; (3) CSRP + L group-CSI, SRP, and Er,Cr:YSGG irradiation; (4) SRP group-manual SRP; (5) L group-Er,Cr:YSGG irradiation; (6) SRP + L group-SRP and Er,Cr:YSGG irradiation. At 7, 15, and 30 days after treatments, animals were euthanized and histologic, histometric, immunohistochemistry, and real-time PCR analyses were performed. Histometrically, no differences were observed in the SRP, L, and SRP + L groups exposed to CSI. The CSRP group showed more bone formation at 30 days than at 15 days (p < 0.01) but less bone at 30 days than the CL group at 30 days (p < 0.05). Immunohistochemical staining was positive for osteoblasts, fibroblasts, and osteoclasts. Real-time PCR showed more (vascular endothelial growth factor) VEGF expression in the L (p < 0.05) and SRP + L (p < 0.01) groups at 30 days than at 15 days and less VEGF expression in the CSRP group at 30 days than at 15 days (p < 0.05). There was no difference in fibroblast growth factor (FGF) expression. The Er,Cr:YSGG laser irradiation promotes favorable conditions for tissue repair even in animals exposed to CSI, with similar results as those achieved from manual scaling and root planing. (AU)

• FAPESP's process: 10/16661-8 - Effects of Er,Cr:YSGG laser in critical bone defects and periodontal disease in rats subjected to cigarette smoke inhalation
• Grantee: Rosemary Adriana Chierici Marcantonio
• Support Opportunities: Regular Research Grants
• FAPESP's process: 09/17639-9 - Evaluation of the effects of Laser ER, CR: YSGG in critical bone defects and in the treatment of induced periodontal disease in rats submitted to inhalation of cigarette smoke
• Grantee: Chaine Pavone
• Support Opportunities: Scholarships in Brazil - Doctorate