Addition of Pentoxifylline to Skim Milk-Based Extender on Frozen-Thawed Equine Sperm

Author(s)

Tsunoda, Roberta Harue ^[1] ; Arruda, Rubens Paes ^[2] ; Serrano-Recalde, Elena Carolina ^[1] ; Martins Oliveira, Bruna Marcele ^[1] ; Florez-Rodriguez, Shirley Andrea ^[1] ; Rodrigues Alves, Maira Bianchi ^[1] ; Lanconi, Renata ^[2] ; Nichi, Marcilio ^[3] ; Carvalho Celeghini, Eneiva Carla ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, Lab Teaching & Res Pathol Reprod, Ctr Biotechnol A, BR-13635900 Pirassununga, SP - Brazil

^[2] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, Lab Semen Biotechnol & Androl, Ctr Biotechnol Anim, BR-13635900 Pirassununga, SP - Brazil

^[3] Univ Sao Paulo, Dept Anim Reprod, Sch Vet Med, Sao Paulo - Brazil

• Document type: Journal article
• Source: Journal of Equine Veterinary Science; v. 35, n. 10, p. 823-829, OCT 2015.
• Web of Science Citations: 0

Abstract

Cryopreservation impairs sperm fertilizing ability by decreasing sperm motility and motion characteristics; therefore, pentoxifylline (PTX) has been used to stimulate sperm motility. We investigated the effects induced by adding PTX to seminal extender on sperm motion characteristics, membranes, and chromatin integrity and sperm morphology of thawed equine sperm. Eight batches from a unique stallion were cryopreserved (200 x 10(6) spermatozoa per mL), thawed, evaluated, and divided in two aliquots: one of them was diluted (1:2) in skim milk extender, and the other was diluted (1:2) in the same skim milk extender + PTX (7.18 mM). Analyses were done at 5 (TO), 30 (T30), 60 (T60), and 120 (T120) minutes after dilution and incubation. Sperm movement was evaluated using the computer-assisted sperm analysis, integrity of plasma and acrosomal membranes, and mitochondria] membrane potential, using fluorescent probes and chromatin denaturation by toluidine blue. Sperm dilution, without or with PTX, improves progressive motility, acrosome integrity, and mitochondria] function; however, the addition of PT)( does not change these. The addition of PTX to skim milk extender increases sperm velocity characteristics, lateral head displacement, and beat-cross frequency but does not affect the motility, cellular membranes, chromatin integrity, and abnormal sperm morphology when used to dilute the frozen-thawed equine sperm. (C) 2015 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 12/14927-6 - Pregnancy rate, inflamatory response and uterine blood flow affected by the effect of Pentoxifylline and sêmen quality assessed by fluorecent probes
• Grantee: Roberta Harue Tsunoda
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 12/15736-0 - Pregnancy rate, inflammatory response and uterine blood flow affected by pentoxifylline and semen quality assessed in equines
• Grantee: Eneiva Carla Carvalho Celeghini
• Support Opportunities: Regular Research Grants