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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Elimination of the artefact peaks in capillary electrophoresis determination of glutamate by using organic solvents in sample preparation

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Author(s):
Madeira Campos, Camila Dalben [1] ; de Campos Braga, Patricia Aparecida [2] ; Reyes Reyes, Felix Guillermo [2] ; Fracassi da Silva, Jose Alberto [3, 1]
Total Authors: 4
Affiliation:
[1] Univ Estadual Campinas, Inst Chem, BR-13803970 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Fac Food Engn, BR-13803970 Campinas, SP - Brazil
[3] Inst Nacl Ciencia & Tecnol Bioanalit INCTBio, Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF SEPARATION SCIENCE; v. 38, n. 21, p. 3781-3787, NOV 2015.
Web of Science Citations: 8
Abstract

Focusing on the demand from the food industry for fast and reliable alternative methods to control the quality of food products, we present in this paper a method for amino acid separation and glutamic acid quantification in complex matrices employing capillary electrophoresis with capacitively coupled contactless conductivity detection. We demonstrate by simulation and experimentally the use of organic solvents in sample preparation to prevent peak splitting and increase stacking in capillary electrophoretic separations of amino acids. Additionally, we obtained results for glutamic acid quantification comparable to those obtained via traditional methods used at industrial sites. We tested premium and low-cost samples with large variations in their glutamic acid content, which demonstrated the wide range of applicability of the method presented herein. The results of the proposed capacitively coupled contactless conductivity detection based capillary electrophoresis method agreed with those obtained by an enzymatic detector and ultra high performance liquid chromatography coupled to tandem mass spectrometry, considering a confidence level of 95%. (AU)

FAPESP's process: 11/02477-3 - Development of a Lab-on-a-chip for glutamate analysis in Foodstuffs
Grantee:Camila Dalben Madeira Campos
Support Opportunities: Scholarships in Brazil - Doctorate