Evaluation of nuclear magnetic resonance spectroscopy variability

Author(s)

Felipe Rodrigues Barreto ^[1] ; Maria Concepción García Otaduy ^[2] ; Carlos Ernesto Garrido Salmon ^[3]

Affiliation

^[1] Universidade de São Paulo. Faculdade de Filosofia Ciências e Letras de Ribeirão Preto - Brasil

^[2] Universidade de São Paulo. Faculdade de Medicina. Departamento de Radiologia - Brasil

^[3] Universidade de São Paulo. Faculdade de Filosofia Ciências e Letras de Ribeirão Preto - Brasil

• Document type: Journal article
• Source: Rev. Bras. Eng. Bioméd.; v. 30, n. 3, p. 242-247, 2014-09-00.

Abstract

INTRODUCTION: The intrinsically high sensitivity of Magnetic Resonance Spectroscopy (MRS) causes considerable variability in metabolite quantification. In this study, we evaluated the variability of MRS in two research centers using the same model of magnetic resonance image scanner. METHODS: Two metabolic phantoms were created to simulate magnetic resonance spectra from in vivo hippocampus. The phantoms were filled with the same basic solution containing the following metabolites: N-acetyl-aspartate, creatine, choline, glutamate, glutamine and inositol. Spectra were acquired over 15 months on 26 acquisition dates, resulting in a total of 130 spectra per center. RESULTS: The phantoms did not undergo any physical changes during the 15-month period. Temporal analysis from both centers showed mean metabolic variations of 3.7% in acquisitions on the same day and of 8.7% over the 15-month period. CONCLUSION: The low deviations demonstrated here, combined with the high specificity of Magnetic Resonance Spectroscopy, confirm that it is feasible to use this technique in multicenter studies in neuroscience research. (AU)

• FAPESP's process: 09/00270-2 - In vitro and in vivo brain metabolites quantification using Magnetic Resonance Spectroscopy
• Grantee: Felipe Rodrigues Barreto
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 05/56464-9 - Neuroscience Imaging Center at University of São Paulo Medical School
• Grantee: Giovanni Guido Cerri
• Support Opportunities: Inter-institutional Cooperation in Support of Brain Research (CINAPCE) - Thematic Grants