| Full text | |
| Author(s): |
Thiago P. Motta
[1]
;
Adriana Frizzarin
[2]
;
Thamires Martins
[3]
;
Mariana S. Miranda
[4]
;
Juliana R.P. Arcaro
[5]
;
Luiz A. Ambrósio
[6]
;
Claudia R. Pozzi
[7]
Total Authors: 7
|
| Affiliation: | [1] Instituto de Zootecnia. Centro Apta Bovinos de Leite - Brasil
[2] Instituto de Zootecnia. Centro Apta Bovinos de Leite - Brasil
[3] Instituto de Zootecnia. Centro Apta Bovinos de Leite - Brasil
[4] Instituto de Zootecnia. Centro Apta Bovinos de Leite - Brasil
[5] Instituto de Zootecnia. Centro Apta Bovinos de Leite - Brasil
[6] Instituto de Zootecnia. Centro Apta Bovinos de Leite - Brasil
[7] Instituto de Zootecnia. Centro Apta Bovinos de Leite - Brasil
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | Pesquisa Veterinária Brasileira; v. 35, n. 1, p. 23-28, 2015-01-00. |
| Abstract | |
The quality of the diet offered to lactating cows is a concern to health officials the possibility of detecting mycotoxins harmful to human and animal health. The objectives were to evaluate the profile of mycoflora, determine the water activity (Aw) and the natural occurrence of aflatoxin B1 (AFB1) in diets offered to lactating cows from dairy farms in the state of São Paulo, Brazil. Samples of the diets were taken directly from the troughs batch of 15 cows, on two consecutive days at intervals of 24 hours and every 15 days with a period of 45 sampling days per farm. Purification and determination of AFB1 were performed on immunoaffinity columns and High Performance Liquid Chromatography (HPLC). The study of mycobiota present in samples of diets (288) revealed that yeast cells were predominant in all diets (83.97 to 99.98%). 15 genera were isolated from filamentous fungi, with Aspergillus spp (20.09%), Fusarium spp. (14.16%) and Penicillium spp. (11.48%) the most prevalent. The counts of colony forming units per gram of food (UFC.g-1) ranged from 102 a1011. The water activity of the samples ranged from 0.91 to 0.98. We have detected the presence of AFB1 in 31.44% of samples with levels between 1.68 a 194.51μg.kg-1. Measures of good production, storage and use should be taken to reduce the occurrence of aflatoxin B1 in the diet offered to lactating cow. (AU) | |
| FAPESP's process: | 08/05428-0 - Study of the factors related to the occurrence of aflatoxin M1 in milk in producting microregions in the State of São Paulo |
| Grantee: | Claudia Rodrigues Pozzi |
| Support Opportunities: | Regular Research Grants |