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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of the fungitoxic activity and isolation of alloaromadendrene - 4α, 10 β - diol in Hypericum cordatum

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Author(s):
N. Scalco [1] ; A.M. Ladeira [2] ; J.H.G. Lago [3] ; M.C.M. Young [4] ; L.R. Carvalho [5]
Total Authors: 5
Affiliation:
[1] Instituto de Botânica. Núcleo de Pesquisa em Fisiologia e Bioquímica - Brasil
[2] Instituto de Botânica. Núcleo de Pesquisa em Fisiologia e Bioquímica - Brasil
[3] Universidade Federal de São Paulo. Departamento de Ciências Exatas e da Terra - Brasil
[4] Instituto de Botânica. Núcleo de Pesquisa em Fisiologia e Bioquímica - Brasil
[5] Instituto de Botânica. Núcleo de Pesquisa em Fisiologia e Bioquímica - Brasil
Total Affiliations: 5
Document type: Journal article
Source: Revista Brasileira de Plantas Medicinais; v. 16, n. 3, p. 744-749, 2014-00-00.
Abstract

The Hypericum cordatum is a species of the Brazilian Cerrado that was selected in a screening of plants with fungitoxic activities. The aim of this work was to isolate and identify the compounds with antifungal activity in leaf extracts in dichloromethane. For this end, the powder made from the leaves of the plants was submitted to exhaustive extraction with petroleum ether and dichloromethane. The extract in dichloromethane and the active fractions were submitted to bioassay-guided fractionation in Sephadex LH - 20 column, respectively, with the following eluents chloroform:methanol (1:1) and a gradient of hexane:dichlorometane (1:4); dichloromethane:acetone (3:3 and 1:4), methanol and water. Afterward, the fractions that showed some activity were submitted to preparative thin layer chromatography of silica gel GF254 and the material with the greatest mass was submitted to semi-preparative HPLC. The active fraction obtained was analyzed by ¹H NMR, and the main component identified was alloaromadendrene-4α-10β - diol. We may then conclude that this is one of the compounds responsible for the fungitoxic activity of Hypericum cordatum. (AU)

FAPESP's process: 98/05074-0 - Conservation and sustainable use of the plant biodiversity from the Cerrado and the Atlantic Forest: chemical diversity and prospecting for potential drugs
Grantee:Vanderlan da Silva Bolzani
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants