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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The involvement of gonadotropins and gonadal steroids in the ovulatory dysfunction of the potamodromous Salminus hilarii (Teleostei: Characidae) in captivity

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Author(s):
Moreira, Renata Guimaraes [1] ; Honji, Renato Massaaki [1] ; Melo, Renato Garcia [2] ; Narcizo, Amanda de Moraes [1] ; Amaral, Juliane Suzuki [1] ; Araujo, Ronaldo de Carvalho [3] ; Silva Hilsdorf, Alexandre Wagner [2]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Dept Fisiol, Inst Biociencias, BR-05508090 Sao Paulo, SP - Brazil
[2] Univ Mogi das Cruzes, Nucleo Biotecnol, Mogi Das Cruzes, SP - Brazil
[3] Univ Fed Sao Paulo, Dept Biofis, Sao Paulo, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: FISH PHYSIOLOGY AND BIOCHEMISTRY; v. 41, n. 6, p. 1435-1447, DEC 2015.
Web of Science Citations: 5
Abstract

Potamodromous teleosts that require migration to reproduce show dysfunctions that block ovulation and spawning while in captivity. To understand the physiological basis of these reproductive dysfunctions, follicle-stimulating hormone b subunit (fshb) and luteinizing hormone b subunit (lhb) gene expression analyses by real-time quantitative PCR, together with measurements of estradiol (E (2)), 17 alpha-hydroxyprogesterone (17 alpha-OHP) and 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (17 alpha,20 beta-DHP) levels, were carried out throughout the reproductive cycle of the potamodromous Salminus hilarii. The following reproductive stages were evaluated in captive and wild females: previtellogenic (PV), advanced maturation/mature (AM) and regression/spent (REG/SPENT). In the wild females, fshb expression decreased from the PV to the AM stage, and the opposite pattern was detected for E (2), which increased from the PV to the AM stage. fshb was expressed at lower levels in captive than in wild females, and this difference did not change during the reproductive cycle. lhb expression also increased from the PV to the AM stage in both groups, but the wild females at the AM and REG/SPENT stages showed higher lhb expression levels than the captive females. The concentrations of 17 alpha-OHP did not change during the reproductive cycle, and the levels were higher in the captive than in the wild females at all reproductive stages. 17 alpha,20 beta-DHP levels did not change between wild and captive females. However, in captive females, the transition from PV to AM stage was followed by an increase in 17 alpha,20 beta-DHP levels. These data indicate that dysfunctions in the gonadotropins and steroids synthesis pathways cause the ovulation failure in captive S. hilarii. (AU)