Occurrence and molecular characterization of Bartonella spp. and hemoplasmas in neotropical primates from Brazilian Amazon

Author(s)

Bonato, Leticia ^[1] ; Pereira Figueiredo, Mayra Araguaia ^[1] ; Goncalves, Luiz Ricardo ^[1] ; Machado, Rosangela Zacarias ^[1] ; Andre, Marcos Rogerio ^[1]

Affiliation

^[1] Univ Estadual Paulista FCAV UNESP, Fac Ciencias Agr & Vet, Lab Imunoparasitol, Dept Patol Vet, Jaboticabal, SP - Brazil

• Document type: Journal article
• Source: COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES; v. 42, p. 15-20, OCT 2015.
• Web of Science Citations: 8

Abstract

Little is known about the prevalence and genetic diversity of Bartonella spp. and hemoplasmas in nonhuman primates (NHP). The present study aimed to investigate the occurrence of and assess the phylogenetic position of Bartonella spp. and hemoplasma species infecting neetropical NHP from Brazilian Amazon. From 2009 to 2013, a total of 98 blood samples from NHP belonging to the Family Cebidae were collected in the island of Sao Luis, state of Maranhao, of which 87 NHP were from Wild Animal Screening Center (CETAS) in the municipality of Sao Luis, and 11 (9 Sapajus sp. and 2 Saimiri sciureus) were from NHP caught in the Sitio Aguahy Private Reserve. DNA samples were screened by previously described PCR protocols for amplifying Bartonella spp. and Mycoplasma spp. based on nuoG, gltA and 16S rRNA genes, respectively. Purified amplicons were submitted to sequencing and phylogenetic analysis. Bacteremia with one or more Bartonella spp. was not detected in NHP. Conversely, 35 NHP were PCR positive to Mycoplasma spp. The Blastn analysis of seven positive randomly selected sequencing products showed percentage of identity ranging from 95% to 99% with other primates hemoplasmas. The Maximum Likelihood phylogenetic analysis based on a 1510 bp of 16S rRNA gene showed the presence of two distinct clusters, positioned within Mycoplasma haemofelis and Mycoplasma suis groups. The phylogenetic assessment suggests the presence of a novel hemoplasma species in NHP from the Brazilian Amazon. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 14/25946-7 - Molecular detection and characterization of Bartonella spp. in primates in amazon region
• Grantee: Leticia Bonato
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 10/12820-4 - Seroepidemiological and molecular characterization of malarial infection in neotropical primates in the state of Maranhão
• Grantee: Rosangela Zacarias Machado
• Support Opportunities: Regular Research Grants