Effect of phenolic compounds from pretreated sugarcane bagasse on cellulolytic and hemicellulolytic activities

Author(s)

Michelin, Michele ^{[1, 2]} ; Ximenes, Eduardo ^[1] ; Teixeira de Moraes Polizeli, Maria de Lourdes ^[2] ; Ladisch, Michael R. ^[1]

Affiliation

^[1] Purdue Univ, Dept Agr & Biol Engn, Renewable Resources Engn Lab, W Lafayette, IN 47907 - USA

^[2] Univ Sao Paulo, Sch Philosophy Sci & Literature Ribeirao Preto, Dept Biol, Lab Microbiol & Cellular Biol, BR-14040901 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Bioresource Technology; v. 199, p. 275-278, JAN 2016.
• Web of Science Citations: 25

Abstract

This work shows both cellulases and hemicellulases are inhibited and deactivated by water-soluble and acetone extracted phenolics from sugarcane bagasse pretreated at 10% (w/v) for 30 min in liquid hot water at 180 or 200 degrees C. The dissolved phenolics in vacuum filtrate increased from 1.4 to 2.4 g/L as temperature increased from 180 to 200 degrees C. The suppression of cellulose and hemicellulose hydrolysis by phenolics is dominated by deactivation of the beta-glucosidase or beta-xylosidase components of cellulase and hemicellulase enzyme by acetone extract at 0.2-0.65 mg phenolics/mg enzyme protein and deactivation of cellulases and hemicellulases by the water soluble components in vacuum filtrate at 0.05-2 mg/mg. Inhibition was a function of the type of enzyme and the manner in which the phenolics were extracted from the bagasse. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 14/09157-2 - Effect of phenolic compounds derived from pretreated biomass on cellulase and xylanase activities
• Grantee: Michele Michelin
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor