Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of allele-specific expression of the X-linked gene MAO-A in trophectoderm cells of bovine embryos produced by somatic cell nuclear transfer

Full text
Ferreira, A. R. [1, 2] ; Aguiar Filho, L. F. C. [2] ; Sousa, R. V. [2] ; Sartori, R. [3, 1] ; Franco, M. M. [4, 5, 2]
Total Authors: 5
[1] Univ Estadual Sao Paulo Julio de Mesquita Filho, Escola Med Vet & Zootecnia, Dept Reprod Anim & Radiol Vet, Botucatu, SP - Brazil
[2] Embrapa Recursos Genet & Biotecnol, Lab Reprod Anim, Brasilia, DF - Brazil
[3] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Zootecnia, Piracicaba, SP - Brazil
[4] Univ Fed Uberlandia, Inst Genet & Bioquim, BR-38400 Uberlandia, MG - Brazil
[5] Univ Fed Uberlandia, Fac Med Vet, BR-38400 Uberlandia, MG - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Genetics and Molecular Research; v. 14, n. 4, p. 12128-12136, 2015.
Web of Science Citations: 1

Somatic cell nuclear transfer (SCNT) may affect epigenetic mechanisms and alter the expression of genes related to embryo development and X chromosome inactivation (XCI). We characterized allele-specific expression of the X-linked gene monoamine oxidase type A (MAO-A) in the trophectoderm (TF) of embryos produced by SCNT. Total RNA was isolated from individual biopsies (N = 25), and the allele-specific expression assessed by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism. Both paternal and maternal alleles were expressed in the trophectoderm. However, a higher frequency of the mono-allelic expression of a specific allele was observed (N = 17; 68%), with the remaining samples showing the presence of mRNA from both alleles (N = 8; 32%). Considering that MAO-A is subject to XCI in bovine, our results suggest that SCNT may influence XCI because neither an imprinted (mono-allelic expression in all samples) nor a random (presence of mRNA from both alleles in all samples) pattern of XCI was observed in TF. Due to the importance of XCI in mammalian embryo development and its sensitivity to in vitro conditions, X-linked genes subject to XCI are candidates for use in the development of embryo quality molecular markers for assisted reproduction. (AU)

FAPESP's process: 10/07971-3 - Effects of in vitro culture and of nutrition on epigenetic reprogramming in bovine oocytes and embryos
Grantee:Allice Rodrigues Ferreira
Support Opportunities: Scholarships in Brazil - Doctorate