| Full text | |
| Author(s): |
Bonfim-Melo, Alexis
[1]
;
Zanetti, Bianca Ferrarini
[2]
;
Ferreira, Eden Ramalho
[1]
;
Vandoninck, Sandy
[3]
;
Han, Sang Won
[2]
;
Van Lint, Johan
[3]
;
Mortara, Renato Arruda
[1]
;
Bahia, Diana
[4, 1]
Total Authors: 8
|
| Affiliation: | [1] Univ Fed Sao Paulo, EPM UNIFESP, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Sao Paulo - Brazil
[2] Univ Fed Sao Paulo UNIFESP, Interdisciplinary Ctr Gene Therapy CINTERGEN, Sao Paulo - Brazil
[3] Univ Leuven, Dept Cellular & Mol Med, Leuven - Belgium
[4] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Biol Geral, Belo Horizonte, MG - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Cellular Microbiology; v. 17, n. 12, p. 1797-1810, DEC 2015. |
| Web of Science Citations: | 9 |
| Abstract | |
Trypanosoma cruzi extracellular amastigotes (EAs) display unique mechanisms for cell invasion that are highly dependent on host actin filaments. Protein kinase D1 (PKD1) phosphorylates and modulates the activity of cortactin, a key regulator of actin dynamics. We evaluated the role of host cortactin and PKD1 in actin filament dynamics during HeLa cell invasion by EAs. Host cortactin, PKD1 and actin are recruited by EAs based on experiments in fixed and live cells by time lapse confocal microscopy. EAs trigger PKD1 and extracellular signal-regulated kinase 1/2 activation, but not Src family kinases, and selectively phosphorylate cortactin. Heat-killed EAs and non-infective epimastigotes both triggered distinct host responses and did not recruit the molecules studied herein. EA invasion was influenced by depletion or overexpression of host cortactin and PKD1, respectively, suggesting the involvement of both proteins in this event. Collectively, these results show new host cell mechanisms subverted during EA internalization into non-phagocytic cells. (AU) | |
| FAPESP's process: | 10/14190-8 - The role of protein kinase D and cortactin in the cellular invasion by extracellular amastigotes of Trypanosoma cruzi |
| Grantee: | Alexis de Sá Ribeiro do Bonfim de Melo |
| Support Opportunities: | Scholarships in Brazil - Master |