Evolution of cytokine profile during the treatment of cerebral toxoplasmosis in HIV-infected patients

Author(s)

Meira, Cristina da Silva ^[1] ; Pereira-Chioccola, Vera Lucia ^[1] ; Vidal, Jose Ernesto ^[2] ; Motoie, Gabriela ^[1] ; da Costa-Silva, Thais Alves ^[1] ; Gava, Ricardo ^[1] ; Group, Toxoplasma

Affiliation

^[1] Adolfo Lutz Inst, Ctr Parasitol & Micol, BR-01246902 Sao Paulo, SP - Brazil

^[2] Inst Infect Emilio Ribas, Dept Neurol, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF IMMUNOLOGICAL METHODS; v. 426, p. 14-18, NOV 2015.
• Web of Science Citations: 2

Abstract

This study was to follow IFN-gamma, TNF-alpha and IL-10 modulation of peripheral blood mononuclear cells (PBMC) from HIV/cerebral toxoplasmosis patients (CT) during specific treatment. The results were compared with two other groups: HIV patients that had CT at least one year before (P/CT) and individuals with chronic toxoplasmosis (CHR). Blood samples (63) collected from three groups were analyzed. CT, 15 patients (3 blood samples collected one day before Toxoplasma gondii treatment; 7 and 15 days during the treatment). P/CT, 5 patients (one blood sample collected at least, one year after the treatment). CHR, 13 individuals with chronic toxoplasmosis (one blood sample). Cytokine levels were assessed by ELISA after PBMC stimulation with T. gondii antigen. CT patients had low IFN-gamma; discrete increase at 7th and 15th days; and the levels were recovered in cured patients (P/CT). CT patients had high TNF-alpha in the beginning of the treatment. TNF-alpha levels decrease during the treatment (7th and 15th) and in those patients who were treated own. IL-10 levels were almost similar in CT and P/CT groups but low when compared with CHR individuals. The evolution of the infection was correlated to restoration-of IFN-gamma response and a decrease of the inflammation. The evaluation of the immune response can provide valuable information and better monitoring of patients during specific treatment. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 11/13939-8 - Study of a group of fungal and parasitic infections aiming the improvement of techniques of characterization and immune-molecular diagnosis
• Grantee: Vera Lúcia Pereira Chioccola
• Support Opportunities: Regular Research Grants
• FAPESP's process: 09/09168-6 - Toxoplasma gondii excreted-secreted antigens (ESA): Analysis of infected host immune response
• Grantee: Cristina da Silva Meira Strejevitch
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/09496-1 - New diagnostic strategies employed in symptomatic toxoplasmosis
• Grantee: Vera Lúcia Pereira Chioccola
• Support Opportunities: Regular Research Grants