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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Comparison of virulence between Paracoccidioides brasiliensis and Paracoccidioides lutzii using Galleria mellonella as a host model

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Scorzoni, Liliana [1] ; Alves de Paula e Silva, Ana Carolina ; Singulani, Junya de Lacorte ; Leite, Fernanda Sangalli ; de Oliveira, Haroldo Cesar ; Moraes da Silva, Rosangela Aparecida ; Fusco-Almeida, Ana Marisa ; Soares Mendes-Giannini, Maria Jose
Total Authors: 8
[1] UNESP Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Anal Clin, Araraquara, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: VIRULENCE; v. 6, n. 8, p. 766-776, NOV 17 2015.
Web of Science Citations: 13

Paracoccidioidomycosis is a systemic mycosis, endemic in Latin America. The etiologic agents of this mycosis are composed of 2 species: Paracoccidioides brasiliensis and P. lutzii. Murine animal models are the gold standard for in vivo studies; however, ethical, economical and logistical considerations limit their use. Galleria mellonella is a suitable model for in vivo studies of fungal infections. In this study, we compared the virulence of P. brasiliensis and P. lutzii in G. mellonella model. The deaths of larvae infected with P. brasiliensis or P. lutzii were similar, and both species were able to reduce the number of hemocytes, which were estimated by microscopy and flow cytometer. Additionally, the phagocytosis percentage was similar for both species, but when we analyze hemocyte-Paracoccidioides spp. interaction using flow cytometer, P. lutzii showed higher interactions with hemocytes. The gene expression of gp43 as well as this protein was higher for P. lutzii, and this expression may contribute to a greater adherence to hemocytes. These results helped us evaluate the behavior of Paracoccidioides spp in G. mellonella, which is a convenient model for investigating the host-Paracoccidioides spp. interaction. (AU)

FAPESP's process: 15/03700-9 - Alternative animal as a model to study Paracoccidioides- host interaction: virulence, efficacy and toxicology of antifungal compounds and new preventive treatments
Grantee:Maria José Soares Mendes Giannini
Support type: Regular Research Grants
FAPESP's process: 13/10917-9 - Alternative animal models: Virulence of different phylogenetic species of Paracoccidioides and effect of the 30kDa 43kDa protein and their antibodies
Grantee:Liliana Scorzoni
Support type: Scholarships in Brazil - Post-Doctorate