Identification of acid-resistant proteins in acquired enamel pellicle

Author(s)

Delecrode, Taisa Ribas ^[1] ; Siqueira, Walter Luiz ^[2] ; Zaidan, Flavia Cardoso ^{[1, 2]} ; Bellini, Melina Rodrigues ^[1] ; Moffaa, Eduardo Buozi ^{[1, 2]} ; Martins Mussi, Maria Carolina ^{[2, 3]} ; Xiao, Yizhi ^[2] ; Rabelo Buzalaf, Marilia Afonso ^[1]

Affiliation

^[1] Univ Sao Paulo, Bauru Sch Dent, BR-17012901 Bauru, SP - Brazil

^[2] Univ Western Ontario, Schulich Sch Med & Dent, London, ON - Canada

^[3] Univ Sao Paulo, Sch Dent, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Journal of Dentistry; v. 43, n. 12, p. 1470-1475, DEC 2015.
• Web of Science Citations: 17

Abstract

Objectives: This study characterized the proteome profile of the acquired pellicle formed in vivo on enamel. Changes in this proteome profile after exposure to lactic or citric acid were also evaluated. Methods: Volunteers (n = 8) were subjected to dental prophylaxis. After 2 h to allow the formation of the acquired pellicle, the teeth were isolated with cotton rolls and 1 mL of citric acid (1%, pH 2.5) or lactic acid (0.1 M pH 4.8) or deionized water was gently applied with a pipette on the anterior teeth (both maxillary and mandibular) for 10 s. In sequence, the pellicle was collected with an electrode filter paper soaked in 3% citric acid. This procedure was repeated for two additional days following a crossover protocol. Proteins were subjected to reverse phase liquid chromatography coupled to mass spectrometry (nLC-ESI-MS/MS). MS/MS data were processed and submitted to Proteome Discoverer software. Searches were done using SWISS-PROT and TrEMBL databases for human proteins. Results: In total, seventy-two proteins were present in all groups and were submitted to quantitative analysis (SIEVE). Some of these proteins were increased more than two-fold after exposure to the acids. Among them, cystatin-B was increased 20- and 13-fold after exposure to citric and lactic acids, respectively. Additionally, some proteins were identified in only one of the groups (18, 5, and 11 proteins for deionized water, citric and lactic acids, respectively). Conclusions: Our results open new insights regarding potentially acid-resistant proteins that could be added to dental products to prevent acidic dissolution of the teeth. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 09/53852-9 - Acquisition of a hybridmass spectrometer with quadrupole and time-of-flight analyzers to identify peptides, proteins and metabolic products
• Grantee: Marília Afonso Rabelo Buzalaf
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 10/15216-0 - Determination of the composition of the acquired pellicle formed on enamel and dentin and of its modification after acid exposure: proteomic study
• Grantee: Marília Afonso Rabelo Buzalaf
• Support Opportunities: Regular Research Grants