The applications of mass spectrometry in biomedical sciences
| Grant number: | 09/53852-9 |
| Support type: | Multi-user Equipment Program |
| Duration: | October 01, 2010 - September 30, 2013 |
| Field of knowledge: | Health Sciences - Dentistry |
| Principal Investigator: | Marília Afonso Rabelo Buzalaf |
| Grantee: | Marília Afonso Rabelo Buzalaf |
| Home Institution: | Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil |
| EMU web page: | Página do Equipamento Multiusuário não informada |
| Use scheduling: | E-mail de agendamento não informado |
Abstract
This FAPESP Multi-User Equipment Program proposal is aimed at the acquisition of a mass spectrometer with nanoelectrospray ionization and two mass analyzers, one quadrupole and one time-of-flight. This equipment is coupled to an ultra-performance liquid chromatograph (UPLC), which allows high-resolution chromatographic separations due to the small inner diameter of its columns. After the separation step, the UPLC injects the samples into the mass spectrometer. An important distinguishing feature of the equipment requested is the ionization system (nanoelectrospray), which allows injection of nanoliters of sample, increasing the sensitivity of the equipment and hence the likelihood of identifying the analyte. This configuration gives the equipment broad applicability in diverse fields such as physics, chemistry, medicine, pharmacy, dentistry, geology, nuclear sciences, material sciences, archeology, petroleum industry, environmental science and forensic science, because its high resolution allows the identification of small molecules as well as intact proteins. Therefore, it is quite versatile and can be used by professionals in proteomics in general, as well as by researchers in other fields, which makes it the ideal multi-user equipment, as evidenced by the diversity of lines of research involved in the proposal. The acquisition of this equipment is strategic because the resulting analyses will constitute a major step toward increasing the flow of projects involving proteomic analysis. That flow has been limited due to a shortage of equipment with the appropriate configuration for such analysis, as well as to the high rate of maintenance of these devices, which are highly sensitive. The equipment will be installed in the 7th administrative region of the state of São Paulo (the city of Bauru), and will serve associated projects underway in regions 8 (Sao Jose do Rio Preto) and 4 (Botucatu), where there are no mass spectrometers of any type, as well as other interested parties who satisfy the conditions of the management of use plan presented. (AU)