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Acquisition of a hybridmass spectrometer with quadrupole and time-of-flight analyzers to identify peptides, proteins and metabolic products

Grant number: 09/53852-9
Support type:Multi-user Equipment Program
Duration: October 01, 2010 - September 30, 2013
Field of knowledge:Health Sciences - Dentistry - Social and Preventive Dentistry
Principal researcher:Marília Afonso Rabelo Buzalaf
Grantee:Marília Afonso Rabelo Buzalaf
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
As informações de acesso ao Equipamento Multiusuário são de responsabilidade do Pesquisador responsável
EMU web page: Página do Equipamento Multiusuário não informada
Type of equipment: Tipo de Equipamento Multiusuário não informado
Manufacturer: Fabricante não informado
Model: Modelo não informado


This FAPESP Multi-User Equipment Program proposal is aimed at the acquisition of a mass spectrometer with nanoelectrospray ionization and two mass analyzers, one quadrupole and one time-of-flight. This equipment is coupled to an ultra-performance liquid chromatograph (UPLC), which allows high-resolution chromatographic separations due to the small inner diameter of its columns. After the separation step, the UPLC injects the samples into the mass spectrometer. An important distinguishing feature of the equipment requested is the ionization system (nanoelectrospray), which allows injection of nanoliters of sample, increasing the sensitivity of the equipment and hence the likelihood of identifying the analyte. This configuration gives the equipment broad applicability in diverse fields such as physics, chemistry, medicine, pharmacy, dentistry, geology, nuclear sciences, material sciences, archeology, petroleum industry, environmental science and forensic science, because its high resolution allows the identification of small molecules as well as intact proteins. Therefore, it is quite versatile and can be used by professionals in proteomics in general, as well as by researchers in other fields, which makes it the ideal multi-user equipment, as evidenced by the diversity of lines of research involved in the proposal. The acquisition of this equipment is strategic because the resulting analyses will constitute a major step toward increasing the flow of projects involving proteomic analysis. That flow has been limited due to a shortage of equipment with the appropriate configuration for such analysis, as well as to the high rate of maintenance of these devices, which are highly sensitive. The equipment will be installed in the 7th administrative region of the state of São Paulo (the city of Bauru), and will serve associated projects underway in regions 8 (Sao Jose do Rio Preto) and 4 (Botucatu), where there are no mass spectrometers of any type, as well as other interested parties who satisfy the conditions of the management of use plan presented. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DELECRODE, TAISA RIBAS; SIQUEIRA, WALTER LUIZ; ZAIDAN, FLAVIA CARDOSO; BELLINI, MELINA RODRIGUES; MOFFAA, EDUARDO BUOZI; MARTINS MUSSI, MARIA CAROLINA; XIAO, YIZHI; RABELO BUZALAF, MARILIA AFONSO. Identification of acid-resistant proteins in acquired enamel pellicle. Journal of Dentistry, v. 43, n. 12, p. 1470-1475, . (09/53852-9, 10/15216-0)
DELECRODE, TAISA RIBAS; SIQUEIRA, WALTER LUIZ; ZAIDAN, FLAVIA CARDOSO; BELLINI, MELINA RODRIGUES; LEITE, ALINE LIMA; XIAO, YISHI; RIOS, DANIELA; MAGALHAES, ANA CAROLINA; RABELO BUZALAF, MARILIA AFONSO. Exposure to acids changes the proteomic of acquired dentine pellicle. Journal of Dentistry, v. 43, n. 5, p. 583-588, . (09/53852-9, 10/15216-0, 12/11083-1, 11/03284-4)

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