| Full text | |
| Author(s): |
Gabriel, Heloisa B.
[1]
;
de Azevedo, Mauro F.
[1]
;
Palmisano, Giuseppe
[1]
;
Wunderlich, Gerhard
[1]
;
Kimura, Emilia A.
[1]
;
Katzin, Alejandro M.
[1]
;
Alves, Joao M. P.
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, Sao Paulo - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | SCIENTIFIC REPORTS; v. 5, DEC 21 2015. |
| Web of Science Citations: | 0 |
| Abstract | |
Malaria is a tropical disease with significant morbidity and mortality. A better understanding of the metabolism of its most important etiological agent, Plasmodium falciparum, is paramount to the development of better treatment and other mitigation measures. Farnesyldiphosphate synthase/geranylgeranyldiphosphate synthase (FPPS/GGPPS) is a key enzyme in the synthesis of isoprenic chains present in many essential structures. In P. falciparum, as well as a handful of other organisms, FPPS/GGPPS has been shown to be a bifunctional enzyme. By genetic tagging and microscopy, we observed a changing localization of FPPS/GGPPS in blood stage parasites. Given the great importance of alternative splicing and other transcriptional phenomena in gene regulation and the generation of protein diversity, we have investigated the processing of the FPPS/GGPPS transcript in P. falciparum by high-throughput sequencing methods in four time-points along the intraerythrocytic cycle of P. falciparum. We have identified levels of transcript diversity an order of magnitude higher than previously observed in this organism, as well as a few stage-specific splicing events. Our data suggest that alternative splicing in P. falciparum is an important feature for gene regulation and the generation of protein diversity. (AU) | |
| FAPESP's process: | 13/14622-3 - Comparative genomics of Trypanosomatidae |
| Grantee: | João Marcelo Pereira Alves |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 10/19518-1 - Functional characterization of farnesyl pyrophosphate synthase (FPPs) and 1,4-dihydroxy-2-naftoato preniltransferase (MenA) respectively involved in the isoprenoid pathway and the vitamin K during the intra-erythrocytic development of P. falciparum |
| Grantee: | Heloisa Berti Gabriel |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 14/23417-7 - Biosynthesis of isoprenoids in Plasmodium falciparum: evaluation of possible targets for to obtain new anti-malarial drugs |
| Grantee: | Alejandro Miguel Katzin |
| Support Opportunities: | Regular Research Grants |