Serum lipidomic profiling as a useful tool for screening potential biomarkers of hepatitis B-related hepatocellular carcinoma by ultraperformance liquid chromatography-mass spectrometry

Author(s):Show less -

Passos-Castilho, Ana Maria ^[1] ; Carvalho, Valdemir Melechco ^[2] ; Morais Cardozo, Karina Helena ^[2] ; Kikuchi, Luciana ^[3] ; Chagas, Aline Lopes ^[3] ; Gomes-Gouvea, Michele Soares ^[3] ; Malta, Fernanda ^[3] ; de Seixas-Santos Nastri, Ana Catharina ^[4] ; Rebello Pinho, Joao Renato ^[3] ; Carrilho, Flair Jose ^[3] ; Hernandes Granato, Celso Francisco ^{[2, 1]}

Affiliation

^[1] Univ Fed Sao Paulo, Div Infect Dis, BR-04039032 Sao Paulo, SP - Brazil

^[2] Fleury Grp, Sao Paulo, SP - Brazil

^[3] Hosp Clin Sao Paulo, Inst Canc Estado Sao Paulo, Sao Paulo Clin Liver Canc Grp, Dept Gastroenterol, Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Sch Med, Dept Infect Dis, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: BMC CANCER; v. 15, DEC 18 2015.
• Web of Science Citations: 6

Abstract

Background: Chronic hepatitis B (CHB) virus infection is a major cause of hepatocellular carcinoma (HCC), as late diagnosis is the main factor for the poor survival of patients. There is an urgent need for accurate biomarkers for early diagnosis of HCC. The aim of the study was to explore the serum lipidome profiles of hepatitis B-related HCC to identify potential diagnostic biomarkers. Methods: An ultraperformance liquid chromatography mass spectrometry (UPLC-MS) lipidomic method was used to characterize serum profiles from HCC (n = 32), liver cirrhosis (LC) (n = 30), CHB (n = 25), and healthy subjects (n = 34). Patients were diagnosed by clinical laboratory and imaging evidence and all presented with CHB while healthy controls had normal liver function and no infectious diseases. Results: The UPLC-MS-based serum lipidomic profile provided more accurate diagnosis for LC patients than conventional alpha-fetoprotein (AFP) detection. HCC patients were discriminated from LC with 78 % sensitivity and 64 % specificity. In comparison, AFP showed sensitivity and specificity of 38 % and 93 %, respectively. HCC was differentiated from CHB with 100 % sensitivity and specificity using the UPLC-MS approach. Identified lipids comprised glycerophosphocolines, glycerophosphoserines and glycerophosphoinositols. Conclusions: UPLC-MS lipid profiling proved to be an efficient and convenient tool for diagnosis and screening of HCC in a high-risk population. (AU)

• FAPESP's process: 13/03701-0 - Identification of lipidomic markers with diagnostic potential in hepatocellular carcinoma
• Grantee: Ana Maria Passos
• Support Opportunities: Scholarships in Brazil - Doctorate