Yeasts of the genital region of patients attending the dermatology service at Hospital Sao Paulo, Brazil

Author(s)

Levy Bentubo, Henri Donnarumma ^[1] ; Mantovani, Ariane ^[2] ; Yamashita, Jane Tomimori ^[3] ; Gambale, Walderez ^[4] ; Fischman, Olga ^[5]

Affiliation

^[1] Univ Cruzeiro do Sul UNICSUL, Ctr Hlth Sci, Sao Paulo - Brazil

^[2] Fed Univ Sao Paulo UNIFESP EPM, Dept Microbiol Immunol & Parasitol, Dept Cell Biol, Sao Paulo, SP - Brazil

^[3] Fed Univ Sao Paulo UNIFESP EPM, Dept Dermatol, Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Dept Microbiol, BR-09500900 Sao Paulo, SP - Brazil

^[5] Fed Univ Sao Paulo UNIFESP EPM, Dept Microbiol Immunol & Parasitol, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: REVISTA IBEROAMERICANA DE MICOLOGIA; v. 32, n. 4, p. 229-234, OCT 31 2015.
• Web of Science Citations: 0

Abstract

Background: The knowledge of the diversity of yeasts that make up the skin microbiota of human beings is essential for the efficient monitoring of infections to which a person may be predisposed. Aims: This study identified yeasts comprising the genital skin microbiota of patients attending the Dermatology Service at the Hospital Sao Paulo-UNIFESP, Brazil. Methods: Samples were collected from the genital region of each patient and cultured on Sabouraud dextrose agar. Individual colonies were carefully transferred to tubes daily. Yeasts were identified based on classical methodologies and confirmed using a commercial kit. Results: Eighty-three patients were included in the study. Approximately 80% were women and 20% were men. The average age was 55 years. Hypertension, diabetes, kidney transplant and AIDS were the main underlying diseases reported by the patients. The most prevalent yeasts were Candida parapsilosis (36.1%), Rhodotorula mucilaginosa (9.2%), Rhodotorula glutinis (8.3%), Candida tropicalis (5.5%) and Trichosporon inkin (1.8%). Approximately 78% of the isolates were obtained in pure cultures. Trichosporon inkin was isolated only from women, in contrast to literature describing a high prevalence in males. Conclusions: Our results suggest that Candida albicans is not the main yeast found on genital skin as previously thought, and opportunistic pathogens such as C. parapsilosis, C. tropicalis, Rhodotorula spp. and T. inkin make up the genital skin microbiota, representing a risk for infection in immunocompromised subjects. These results also indicate that women are carriers of T. inkin, the etiological agent of white piedra and trichosporonosis. (C) 2013 Revista Iberoamericana de Micologia. Published by Elsevier Espana, S.L.U. All rights reserved. (AU)