Evaluation of different pig oral mucosa sites as permeability barrier models for drug permeation studies

Author(s)

Franz-Montan, Michelle ^[1] ; Serpe, Luciano ^[1] ; Maia Martinelli, Claudia Cristina ^[1] ; da Silva, Camila Batista ^[1] ; dos Santos, Cleiton Pita ^[1] ; Novaes, Pedro Duarte ^[2] ; Volpato, Maria Cristina ^[1] ; de Paula, Eneida ^[3] ; Vianna Lopez, Renata Fonseca ^[4] ; Groppo, Francisco Carlos ^[1]

Affiliation

^[1] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Physiol Sci, Piracicaba, SP - Brazil

^[2] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Morphol, Piracicaba, SP - Brazil

^[3] Univ Campinas UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, Campinas, SP - Brazil

^[4] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, BR-14049 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: European Journal of Pharmaceutical Sciences; v. 81, p. 52-59, JAN 1 2016.
• Web of Science Citations: 8

Abstract

The objective of the present study was to investigate the influence of preparation and storage conditions on the histology and permeability of different parts of porcine oral mucosa used for in vitro studies of transbuccal formulations. Fresh and frozen (-20 degrees C and -80 degrees C, with or without cryoprotectant) epithelia of porcine palatal, gingival, dorsum of the tongue, and buccal mucosa were submitted for histological analyses to determine the effects of storage conditions on barrier integrity. Permeation of lidocaine hydrochloride (used as a hydrophilic model drug) across fresh and previously frozen oral epithelium was measured in order to evaluate the barrier function. Histological evaluation demonstrated that the oral epithelium was successfully separated from the connective tissue, except for gingival mucosa. After storage under different conditions, all tissues presented desquamation of superficial layers and spherical spaces induced by the freezing process. The permeability of lidocaine hydrochloride varied among the fresh oral mucosa and generally increased after freezing. In conclusion, fresh epithelium from the buccal and dorsum of the tongue mucosa should be used for in vitro studies investigating hydrophilic drug transport when these are the desired clinical application sites. However, when the palate is the target site, both fresh and frozen (for up to 4 weeks, without addition of cryoprotectant) samples could be used. The addition of glycerol as a cryoprotectant should be avoided due to increased lidocaine hydrochloride permeability. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 11/23475-9 - Permeation studies of lidocaine across mucosal oral epithelium
• Grantee: Claudia Cristina Maia Martinelli
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 12/06974-4 - Development, biocompatibility and permeation studies on gel formulations of poly-epsilon-caprolactone nanocapsules containing local anesthetics
• Grantee: Michelle Franz Montan Braga Leite
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 11/18113-0 - Development of methods to perform permeation studies of topical formulations for dentistry applications
• Grantee: Francisco Carlos Groppo
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/14457-5 - Lipid-based nanocarriers (SLN/NLC and remote-loading liposomes) used to improve the upload and potency of local anesthetics
• Grantee: Eneida de Paula
• Support Opportunities: Research Projects - Thematic Grants