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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of low level laser therapy on inflammatory and angiogenic gene expression during the process of bone healing: A microarray analysis

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Tim, Carla Roberta [1] ; Bossini, Paulo Sergio [2] ; Kido, Hueliton Wilian [1] ; Malavazi, Iran [3] ; von Zeska Kress, Marcia Regina [4] ; Carazzolle, Marcelo Falsarella [5, 6] ; Parizotto, Nivaldo Antonio [1] ; Renno, Ana Claudia [2]
Total Authors: 8
[1] Univ Fed Sao Carlos, Dept Physiotherapy, Rod Washington Luis Km 235, BR-13565905 Sao Carlos, SP - Brazil
[2] Univ Fed Sao Paulo, Dept Biosci, Ave Ana Costa 95, BR-11050240 Santos - Brazil
[3] Univ Fed Sao Carlos, Dept Genet & Evolut, Rod Washington Luis Km 235, BR-13565905 Sao Carlos, SP - Brazil
[4] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Bromatol, Ave Cafe 95, BR-14049900 Ribeirao Preto - Brazil
[5] Univ Estadual Campinas, Dept Genet & Evolut, Cidade Univ Zeferino Vaz, BR-13083970 Campinas, SP - Brazil
[6] Brazilian Natl Ctr Res Energy & Mat, Biosci Natl Lab, Giuseppe Maximo Scolfaro 10-000, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Web of Science Citations: 9

The process of bone healing as well as the expression of inflammatory and angiogenic genes after low level laser therapy (LLLT) were investigated in an experimental model of bone defects. Sixty Wistar rats were distributed into control group and laser group (830 nm, 30 mW, 2,8 J, 94 seg). Histopathological analysis showed that LLLT was able to modulate the inflammatory process in the area of the bone defect and also to produce an earlier deposition of granulation tissue and newly formed bone tissue. Microarray analysis demonstrated that LLLT produced an up-regulation of the genes related to the inflammatory process (MMD, PTGIR, PTGS2, Ptger2, ILL 1IL6, IL8, IL18) and the angiogenic genes (FGF14, FGF2, ANGPT2, ANGPT4 and PDGFD) at 36 h and 3 days, followed by the decrease of the gene expression on day 7. Immunohistochemical analysis revealed that the subjects that were treated presented a higher expression of COX-2 at 36 h after surgery and an increased VEGF expression on days 3 and 7 after surgery. Our findings indicate that LLLT was efficient on accelerating the development of newly formed bone probably by modulating the inflammatory and angiogenic gene expression as well as COX2 and VEGF immunoexpression during the initial phase of bone healing. (C) 2015 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 10/15335-0 - Analysis of gene expression during bone repair defects in tibia irradiated with low intensity laser therapy
Grantee:Carla Roberta Tim
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/08293-7 - CCES - Center for Computational Engineering and Sciences
Grantee:Munir Salomao Skaf
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC