fosI Is a New Integron-Associated Gene Cassette Encoding Reduced Susceptibility to Fosfomycin

Author(s)

Pelegrino, Karla de Oliveira ^[1] ; Campos, Juliana Coutinho ^[2] ; Ferreira Sampaio, Suely Carlos ^[3] ; Lezirovitz, Karina ^[4] ; Seco, Bruna Mara ^[2] ; Pereira, Mayne de Oliveira ^[2] ; da Costa Rocha, Darlan Augusto ^[2] ; Jove, Thomas ^[5] ; Nicodemo, Antonio Carlos ^[1] ; Mello Sampaio, Jorge Luiz ^{[2, 6]}

Affiliation

^[1] Univ Sao Paulo, Sch Med, Dept Infect Dis, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Sch Pharm, Clin Anal & Toxicol Dept, Sao Paulo, SP - Brazil

^[3] Univ Fed Sao Paulo, Dept Microbiol & Imunol, Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Sch Med, Hosp Clin, Otorhinolaryngol Lab LIM32, Sao Paulo, SP - Brazil

^[5] Univ Limoges, INSERM, CHU Limoges, UMR S 1092, Limoges - France

^[6] Fleury Med & Hlth, Dept Microbiol, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Antimicrobial Agents and Chemotherapy; v. 60, n. 1, p. 686-688, JAN 2016.
• Web of Science Citations: 0

Abstract

In this work, we demonstrate that the fosI gene encodes a predicted small protein with 134 amino acids and determines reduced susceptibility to fosfomycin. It raised the MIC from 0.125 to 6 mu g/ml when the pBRA100 plasmid was introduced into Escherichia coli TOP10 and to 16 mu g/ml when the gene was cloned into the pBC\_SK(-) vector and expressed in E. coli TOP10. (AU)

• FAPESP's process: 10/00807-3 - Phenotypic and genotypic characterization of antimicrobial resistance in rapidly growing mycobacteria
• Grantee: Jorge Luiz Mello Sampaio
• Support Opportunities: Regular Research Grants