Validation of analytical procedures using HPLC-ELSD to determine six sesquiterpene lactones in Eremanthus species

Author(s)

Sousa, Joao Paulo B. ^[1] ; Nogueira, Elidia F. ^[1] ; Ferreira, Leandro S. ^[1] ; Lopes, Norberto P. ^[1] ; Lopes, Joao Luis C. ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Nucleo Pesquisas Prod Nat & Sintet, Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: BIOMEDICAL CHROMATOGRAPHY; v. 30, n. 3, p. 484-493, MAR 2016.
• Web of Science Citations: 1

Abstract

Eremanthus species display sesquiterpene lactones with therapeutic potential. We are proposing the development of a new analytical method that has been completely validated to qualify Eremanthus species and its main lactones in raw material using HPLC-ELSD. For the sample preparation, 10.0mg of powdered Eremanthus leaves was extracted with a 5mL MeOH/H2O (9:1v/v) solution containing scopoletin at 140 mu g/mL as the internal standard. For the separation of eight compounds, six of which were lactones, one internal (IS) and one secondary standard were performed utilizing monolithic columns with a nonlinear gradient. The selectivity, stability, precision and matrix effects parameters showed values of RSD of <10%. The six lactones and scopoletin (IS) were recovered with a proportion between 74 and 90% with accuracy represented by error at -25.41%. The linear dynamic range was obtained between 10.0 and 310.0 mu g/mL for all compounds with r(2)>0.9987. The limits of detection and quantitation ranged from 2.00 to 6.79 mu g/mL and from 6.00 to 20.40 mu g/mL, respectively. Assessing the robustness study, this method can be used in inter-laboratory studies. Using the HPLC-ELSD method, six sesquiterpene lactones including 4,5-dihydro-2,3-dihydroxy-15-deoxy-goyazensolide, goyazensolide, 4,5-dihydro-2,3-epoxy-15-deoxy-goyazensolide, centratherin, 4,5-dihydro-15-deoxy-goyazensolide and lychnofolide, were detected and quantified from distinct Eremanthus species, which were collected in different regions. Copyright (c) 2015 John Wiley \& Sons, Ltd. (AU)

• FAPESP's process: 11/01089-0 - Development and chromatographic method validation to quantify goyazensolide and derivatives in rat plasma
• Grantee: João Paulo Barreto de Sousa
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 09/51812-0 - Development of a platform for the study of in vitro and in vivo metabolism of natural products, a need for pre-clinical testing system
• Grantee: Norberto Peporine Lopes
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants