Proteomic Analysis of Urine in Rats Chronically Exposed to Fluoride

Author(s)

Nakai Kobayashi, Claudia Ayumi ^[1] ; Leite, Aline de Lima ^[1] ; da Silva, Thelma Lopes ^[1] ; dos Santos, Lucilene Delazari ^[2] ; Sousa Nogueira, Fabio Cesar ^[3] ; Santos, Keity Souza ^[4] ; de Oliveira, Rodrigo Cardoso ^[1] ; Palma, Mario Sergio ^[2] ; Domont, Gilberto Barbosa ^[3] ; Rabelo Buzalaf, Marilia Afonso ^[1]

Affiliation

^[1] Univ Sao Paulo, Bauru Dent Sch, Bauru, SP - Brazil

^[2] Univ Estadual Paulista, Rio Claro Biosci Inst, Rio Claro, SP - Brazil

^[3] Univ Fed Rio de Janeiro, Inst Chem, Rio De Janeiro - Brazil

^[4] Univ Sao Paulo, Sao Paulo Med Sch, Discipline Allergy & Immunopathol, Sao Paulo - Brazil

• Document type: Journal article
• Source: JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY; v. 25, n. 1, p. 8-14, 2011.
• Web of Science Citations: 12

Abstract

Urine is an ideal source of materials to search for potential disease-related biomarkers as it is produced by the affected tissues and can be easily obtained by noninvasive methods. 2-DE-based proteomic approach was used to better understand the molecular mechanisms of injury induced by fluoride (F-) and define potential biomarkers of dental fluorosis. Three groups of weanling male Wistar rats were treated with drinking water containing 0 (control), 5, or 50 ppm F- for 60 days (n = 15/group). During the experimental period, the animals were kept individually in metabolic cages, to analyze the water and food consumption, as well as fecal and urinary F excretion. Urinary proteome profiles were examined using 2-DE and Colloidal Coomassie Brilliant Blue staining. A dose-response regarding F- intake and excretion was detected. Quantitative intensity analysis revealed 8, 11, and 8 significantly altered proteins between control vs. 5 ppm F-, control vs. 50 ppm F- and 5 ppm F- vs. 50 ppm F- groups, respectively. Two proteins regulated by androgens (androgen-regulated 20-KDa protein and 0c-2,1-globulin) and one related to detoxification (aflatoxin-Bl-aldehyde-reductase) were identified by MALDI-TOF-TOF MS/MS. Thus, proteomic analysis can help to better understand the mechanisms underlying F- toxicity, even in low doses. 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 25:8-14, 2011; View this article online at wileyonlinelibrary.com. DOI 10:1002/jbt.20353 (AU)

• FAPESP's process: 05/02744-0 - Proteomic analysis in body fluids and soft tissues in rats submitted to treatment with fluoride
• Grantee: Marília Afonso Rabelo Buzalaf
• Support Opportunities: Regular Research Grants