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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Proteome analysis of the plasma protein layer adsorbed to a rough titanium surface

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Author(s):
Dodo, Cindy Goes [1] ; Senna, Plinio Mendes [1] ; Custodio, William [2] ; Paes Leme, Adriana Franco [3] ; Del Bel Cury, Altair Antoninha [1]
Total Authors: 5
Affiliation:
[1] Univ Estadual Campinas, Dept Prosthodont & Periodontol, Piracicaba Dent Sch, Sao Paulo - Brazil
[2] Univ Western Ontario, Dept Biochem, Schulich Sch Med & Dent, London, ON - Canada
[3] Brazilian Biosci Natl Lab LNBio, Mass Spectrometry Lab, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: BIOFOULING; v. 29, n. 5, p. 549-557, MAY 1 2013.
Web of Science Citations: 10
Abstract

In this study a label-free proteomic approach was used to investigate the composition of the layer of protein adsorbed to rough titanium (Ti) after exposure to human blood plasma. The influence of the protein layer on the surface free energy (SFE) of the Ti was evaluated by contact angle measurements. Ti discs were incubated with blood plasma for 180min at 37 degrees C, and the proteins recovered were subjected to liquid chromatography coupled to tandem mass spectrometry analysis. A total of 129 different peptides were identified and assigned to 25 distinct plasma proteins. The most abundant proteins were fibronectin, serum albumin, apolipoprotein A-I, and fibrinogen, comprising 74.54% of the total spectral counts. Moreover, the protein layer increased the SFE of the Ti (p<0.05). The layer adsorbed to the rough Ti surface was composed mainly of proteins related to cell adhesion, molecule transportation, and coagulation processes, creating a polar and hydrophilic interface for subsequent interactions with host cells. (AU)

FAPESP's process: 10/09308-0 - The influence of plasm ion nitriding of titanium surface and colagen type I on serum proteins adsorption on titanium surface, and its influence on adhesion, morphological structuration and bioactivity of human osteoblasts
Grantee:Plinio Mendes Senna
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 10/09113-4 - The influence of plasm ion nitriding of titanium surface and colagen type I on serum proteins adsorption on titanium surface, and its influence on adhesion, morphological structuration and bioactivity of human osteoblasts
Grantee:Altair Antoninha Del Bel Cury
Support Opportunities: Regular Research Grants