Treatment of bowel in experimental gastroschisis with a nitric oxide donor

Author(s)

Goncalves, Frances L. L. ^[1] ; Bueno, Marcia P. ^[2] ; Schmidt, Augusto F. ^[3] ; Figueira, Rebeca L. ^[1] ; Sbragia, Lourenco ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Med Ribeirao Preto, Lab Expt Fetal Surg Michael Harrison, Div Pediat Surg, Dept Surg & Anat, BR-14049 Ribeirao Preto - Brazil

^[2] Univ Estadual Campinas, Sch Med Sci, Dept Obstet & Gynecol, Sao Paulo - Brazil

^[3] Cincinnati Childrens Hosp Med Ctr, Div Neonatol, Sect Neonatol Perinatal & Pulm Biol, Cincinnati, OH 45229 - USA

• Document type: Journal article
• Source: American Journal of Obstetrics and Gynecology; v. 212, n. 3 MAR 2015.
• Web of Science Citations: 2

Abstract

OBJECTIVE: To reduce the harmful effect of bowel exposure to amniotic fluid in gastroschisis, we used the nitric oxide (NO) donor S-nitrosoglutathione (GSNO) in an animal model of gastroschisis and assessed the ideal concentration for treatment of changes in bowel. STUDY DESIGN: Gastroschisis was surgically induced in rat fetuses on day 18.5 of gestation. The fetuses were divided into 5 groups (n = 12 animals/group): control (C), gastroschisis (G), gastroschisis + GSNO 5 mu mol/L (GNO1), gastroschisis + GSNO 0.5 mu mol/L (GNO2), and gastroschisis + GSNO 0.05 mu mol/L (GNO3). On day 21.5 of gestation, fetuses were collected by cesarean delivery. Body and intestinal weight were measured and the bowels were either fixed for histometric and immunohistochemical study or frozen for Western blotting. We analyzed bowel morphometry on histological sections and expression of the NO synthase (NOS) enzymes by Western blotting and immunohistochemistry. Data were analyzed by analysis of variance or Kruskal-Wallis test when appropriate. RESULTS: Morphological and histometric measurements of weight, diameter, and thickness of the layers of the intestinal wall decreased with GSNO treatment, especially in the GNO3 group, when compared with the G group (P < .05). The expression of neuronal NOS, endothelial NOS, and inducible NOS decreased mainly in GNO3 group compared to the G group (P < .05), with no difference compared to C group (P > .05). CONCLUSION: Fetal treatment with 0.05 mmol/L GSNO resulted in significant improvement of bowel morphology in gastroschisis. (AU)

• FAPESP's process: 11/12587-0 - Evaluation in vivo of the effect of corticosteroids and ventilation in oxide nitric pathway on the expression of VEGF and its pulmonary receptors after fetal tracheal occlusion in the congenital diaphragmatic hernia in rats
• Grantee: Frances Lilian Lanhellas Gonçalves
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 11/00794-1 - Assessment of nitric oxide (NO) in vivo after fetal tracheal occlusion on the expression of VEGF receptors in pulmonary congenital diaphragmatic hernia induced by Nitrofen
• Grantee: Lourenço Sbragia Neto
• Support type: Regular Research Grants