Treatment of bowel in experimental gastroschisis with a nitric oxide donor

Autor(es)

Goncalves, Frances L. L. ^[1] ; Bueno, Marcia P. ^[2] ; Schmidt, Augusto F. ^[3] ; Figueira, Rebeca L. ^[1] ; Sbragia, Lourenco ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med Ribeirao Preto, Lab Expt Fetal Surg Michael Harrison, Div Pediat Surg, Dept Surg & Anat, BR-14049 Ribeirao Preto - Brazil

^[2] Univ Estadual Campinas, Sch Med Sci, Dept Obstet & Gynecol, Sao Paulo - Brazil

^[3] Cincinnati Childrens Hosp Med Ctr, Div Neonatol, Sect Neonatol Perinatal & Pulm Biol, Cincinnati, OH 45229 - USA

• Tipo de documento: Artigo Científico
• Fonte: American Journal of Obstetrics and Gynecology; v. 212, n. 3 MAR 2015.
• Citações Web of Science: 2

Resumo

OBJECTIVE: To reduce the harmful effect of bowel exposure to amniotic fluid in gastroschisis, we used the nitric oxide (NO) donor S-nitrosoglutathione (GSNO) in an animal model of gastroschisis and assessed the ideal concentration for treatment of changes in bowel. STUDY DESIGN: Gastroschisis was surgically induced in rat fetuses on day 18.5 of gestation. The fetuses were divided into 5 groups (n = 12 animals/group): control (C), gastroschisis (G), gastroschisis + GSNO 5 mu mol/L (GNO1), gastroschisis + GSNO 0.5 mu mol/L (GNO2), and gastroschisis + GSNO 0.05 mu mol/L (GNO3). On day 21.5 of gestation, fetuses were collected by cesarean delivery. Body and intestinal weight were measured and the bowels were either fixed for histometric and immunohistochemical study or frozen for Western blotting. We analyzed bowel morphometry on histological sections and expression of the NO synthase (NOS) enzymes by Western blotting and immunohistochemistry. Data were analyzed by analysis of variance or Kruskal-Wallis test when appropriate. RESULTS: Morphological and histometric measurements of weight, diameter, and thickness of the layers of the intestinal wall decreased with GSNO treatment, especially in the GNO3 group, when compared with the G group (P < .05). The expression of neuronal NOS, endothelial NOS, and inducible NOS decreased mainly in GNO3 group compared to the G group (P < .05), with no difference compared to C group (P > .05). CONCLUSION: Fetal treatment with 0.05 mmol/L GSNO resulted in significant improvement of bowel morphology in gastroschisis. (AU)

• Processo FAPESP: 11/12587-0 - Avaliação in vivo do efeito do corticóide e da ventilação na via do óxido nítrico sobre a expressão do VEGF e seus receptores pulmonares após a oclusão traqueal fetal na hérnia diafragmática congênita em ratos
• Beneficiário: Frances Lilian Lanhellas Gonçalves
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/00794-1 - Avaliação da via do óxido nítrico in vivo após a oclusão traqueal fetal sobre a expressão de receptores de VEGF pulmonar na hérnia diafragmática congênita em ratos induzida pelo Nitrofen
• Beneficiário: Lourenço Sbragia Neto
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/51487-9 - Avaliacao da protecao das alcas intestinais fetais utilizando hidrogel (biomaterial) e oxido nitrico no modelo experimental de gastrosquise
• Beneficiário: Lourenço Sbragia Neto
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/52772-9 - Efeito do acido retinoico sobre a expressao de receptores de vegf pulmonares em modelos animais de hernia diafragmatica congenita induzida por nitrofen.
• Beneficiário: Augusto Frederico Santos Schmidt
• Modalidade de apoio: Bolsas no Brasil - Mestrado