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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In Vitro Regulation of CCL3 and CXCL12 by Bacterial By-products Is Dependent on Site of Origin of Human Oral Fibroblasts

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Author(s):
Sipert, Carla Renata [1] ; Morandini, Ana Carolina [1] ; Dionisio, Thiago Jose [1] ; Andrade Moreira Machado, Maria Aparecida [2] ; Penha Oliveira, Sandra Helena [3] ; Campanelli, Ana Paula [1] ; Kuo, Winston Patrick [4, 5] ; Santos, Carlos Ferreira [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Sao Paulo - Brazil
[2] Univ Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Community Hlth, Sao Paulo - Brazil
[3] Univ Estadual Paulista, Aracatuba Sch Dent, Dept Basic Sci, Sao Paulo - Brazil
[4] Harvard Univ, Sch Dent Med, Dept Dev Biol, Boston, MA 02115 - USA
[5] Harvard Univ, Sch Med, Harvard Clin & Translat Sci Ctr, Lab Innovat Translat Technol, Boston, MA - USA
Total Affiliations: 5
Document type: Journal article
Source: JOURNAL OF ENDODONTICS; v. 40, n. 1, p. 95-100, JAN 2014.
Web of Science Citations: 8
Abstract

Introduction: Production of chemokines by tissue resident cells is one of the main mechanisms involved in the inflammatory infiltrate formation during inflammation. The specific ability of fibroblasts from different oral tissues such as gingiva, periodontal ligament, and dental pulp from permanent and deciduous teeth in producing the chennokines CCL3 and CXCL12 under stimulation by bacterial products commonly found in endodontic infections was investigated. Methods: Cultures of fibroblasts from gingiva and periodontal ligament as well as from dental pulp from permanent and deciduous teeth were established by using an explant technique and stimulated with increasing concentrations of Escherichia coli lipopolysaccharide (EcLPS) and Enterococcus faecalis lipoteichoic acid (EfLTA) for 1, 6, and 24 hours. Supernatants were tested for CCL3 and CXCL12 by enzyme-linked immunosorbent assay. Results: In general, CCL3 production was induced by EcLPS in the 4 fibroblast subtypes and by EfLTA in fibroblasts from gingiva and periodontal ligament Constitutive CXCL12 synthesis decreased in all fibroblast subtypes especially under stimulation with EcLPS. Fibroblast from permanent deciduous teeth was the cell type presenting the most expressive reduction in CXCL12 release by both stimuli. On the basis of computational matching of CXCL12 mRNA with the microRNAs miR-141 and miR-200a, their expression was also investigated. Although detected in the fibroblasts, these molecules remained unaltered by bacterial by-product stimulation. Conclusions: EcLPS and EfLTA induced the production of CCL3 and unbalanced the synthesis of CXCL12 in a manner dependent on the specific tissue origin of fibroblasts. (AU)

FAPESP's process: 07/00306-1 - Expression of procollagen type I, MIP-1alpha and SDF-1alpha by human gingival fibroblasts and dental pulp stimulated by Enterococcus faecalis lipoteichoic acid and lipopolysaccharide from Escherichia coli and Porphyromonas gingivalis
Grantee:Carla Renata Sipert
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 05/60167-0 - Expression of procollagen type 1, MIP-1± alpha and SDF-1 alpha by human fibroblasts stimulated pulp lipoteichoic acid from Streptococcus mutans
Grantee:Carlos Ferreira dos Santos
Support Opportunities: Regular Research Grants
FAPESP's process: 09/53848-1 - Acquisition of two large-capacity devices (a Milliplex Analyzer Xponent 3 and accessories and a 7900 HT Fast Real Time PCR System) for research conductedat the University of São Paulo School of Dentistry and at other research institutes
Grantee:Carlos Ferreira dos Santos
Support Opportunities: Multi-user Equipment Program