Concentration of Sonication Fluid through Centrifugation Is Superior to Membrane Filtration for Microbial Diagnosis of Orthopedic Implant-Associated Infection

Author(s)

Zitron, Renato ^{[1, 2]} ; Wajsfeld, Tali ^{[1, 2]} ; Klautau, Giselle Burlamaqui ^{[1, 2]} ; da Silva, Cely Barreto ^[3] ; Nigro, Stanley ^[3] ; Mercadante, Marcelo Tomanik ^[4] ; Polesello, Giancarlo Cavalli ^[4] ; Leite Cury, Ricardo de Paula ^[4] ; Costa Salles, Mauro Jose ^{[1, 2]}

Affiliation

^[1] Santa Casa Sao Paulo Sch Med Sci, Div Infect Dis, Sao Paulo - Brazil

^[2] Santa Casa Sao Paulo Sch Med Sci, Dept Internal Med, Sao Paulo - Brazil

^[3] Santa Casa Sao Paulo Sch Med Sci, Dept Lab Med & Pathol, Sao Paulo - Brazil

^[4] Santa Casa Sao Paulo Sch Med Sci, Dept Orthoped Surg, Sao Paulo - Brazil

• Document type: Journal article
• Source: Journal of Clinical Microbiology; v. 54, n. 3, p. 788-790, MAR 2016.
• Web of Science Citations: 1

Abstract

Microbial identification of orthopedic implant-associated infections using sonication fluid (SF) submitted to a concentration step by membrane filtration (SMF) was compared with the standard centrifugation (SC) method. Among 33 retrieved infected implants, sonication identified microorganisms in 26 (78.8%). The sensitivity of SC was higher than that of SMF (78.8% versus 30.3%; P < 0.001). (AU)

• FAPESP's process: 13/00144-2 - Comparison of the membrane filtration of the sonication fluid technique with the habitual centrifugation of the sonication fluid technique for diagnosis of infection in orthopaedic implants biofilms
• Grantee: Renato Zitron
• Support Opportunities: Scholarships in Brazil - Scientific Initiation