High-resolution proteomic profiling of spider venom: expanding the toxin diversity of Phoneutria nigriventer venom

Author(s)

Liberato, Tarcisio ^{[1, 2]} ; Paolo Troncone, Lanfranco Ranieri ^[1] ; Yamashiro, Edson T. ^[3] ; Serrano, Solange M. T. ^[3] ; Zelanis, Andre ^{[2, 3]}

Affiliation

^[1] Inst Butantan, Farmacol Lab, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Lab Prote Func, Dept Ciencia & Tecnol, ICT UNIFESP, Campus Sao Jose dos Campos, Rua Talim 330, BR-12231280 Sao Paulo, SP - Brazil

^[3] Inst Butantan, Lab Especial Toxinol Aplicada, Ctr Toxins Immune Response & Cell Signaling CeTIC, Sao Paulo - Brazil

• Document type: Journal article
• Source: Amino Acids; v. 48, n. 3, p. 901-906, MAR 2016.
• Web of Science Citations: 4

Abstract

Here we present a proteomic characterization of Phoneutria nigriventer venom. A shotgun proteomic approach allowed the identification, for the first time, of O-glycosyl hydrolases (chitinases) in P. nigriventer venom. The electrophoretic profiles under nonreducing and reducing conditions, and protein identification by mass spectrometry, indicated the presence of oligomeric toxin structures in the venom. Complementary proteomic approaches allowed for a qualitative and semi-quantitative profiling of P. nigriventer venom complexity, expanding its known venom proteome diversity. (AU)

• FAPESP's process: 13/07467-1 - CeTICS - Center of Toxins, Immune-Response and Cell Signaling
• Grantee: Hugo Aguirre Armelin
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 11/08514-8 - Study of the degradome of HF3, a PIII-class snake venom metalloproteinase from Bothrops jararaca venom, on cultured fibroblasts
• Grantee: André Zelanis Palitot Pereira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral