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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Xenogeneic Mesenchymal Stromal Cells Improve Wound Healing and Modulate the Immune Response in an Extensive Burn Model

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Author(s):
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Caliari-Oliveira, Carolina [1, 2] ; Ueda Yaochite, Juliana Navarro [1, 2] ; Zambelli Ramalho, Leandra Mira [3] ; Bonini Palma, Patricia Vianna [1] ; Carlos, Daniela [2] ; Cunha, Fernando de Queiroz [4] ; De Souza, Daurea Abadia [5] ; Cipriani Frade, Marco Andrey [6] ; Covas, Dimas Tadeu [6, 1] ; Ribeiro Malmegrim, Kelen Cristina [7, 1] ; Oliveira, Maria Carolina [6, 1] ; Voltarelli, Julio Cesar
Total Authors: 12
Affiliation:
[1] Univ Sao Paulo, Reg Blood Ctr Ribeirao Preto, Ctr Cell Based Therapy CEPID FAPESP, Sao Paulo - Brazil
[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Basic & Appl Immunol Program, Dept Biochem & Immunol, Tenente Catao Roxo 2501, BR-14051140 Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pathol, Sao Paulo - Brazil
[4] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pharmacol, Sao Paulo - Brazil
[5] Univ Fed Uberlandia, Sch Med, Dept Internal Med, Uberlandia, MG - Brazil
[6] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Internal Med, Sao Paulo - Brazil
[7] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Toxicol & Bromatol Anal, Sao Paulo - Brazil
Total Affiliations: 7
Document type: Journal article
Source: CELL TRANSPLANTATION; v. 25, n. 2, p. 201-215, 2016.
Web of Science Citations: 12
Abstract

Major skin burns are difficult to treat. Patients often require special care and long-term hospitalization. Besides specific complications associated with the wounds themselves, there may be impairment of the immune system and of other organs. Mesenchymal stromal cells (MSCs) are a recent therapeutic alternative to treat burns, mainly aiming to accelerate the healing process. Several MSC properties favor their use as therapeutic approach, as they promote angiogenesis, stimulate regeneration, and enhance the immunoregulatory function. Moreover, since patients with extensive burns require urgent treatment and because the expansion of autologous MSCs is a time-consuming process, in this present study we chose to evaluate the therapeutic potential of xenogeneic MSCs in the treatment of severe burns in rats. MSCs were isolated from mouse bone marrow, expanded in vitro, and intradermally injected in the periphery of burn wounds. MSC-treated rats presented higher survival rates (76.19%) than control animals treated with PBS (60.86%, p<0.05). In addition, 60 days after the thermal injury, the MSC-treated group showed larger proportion of healed areas within the burn wounds (90.81 +/- 5.05%) than the PBS-treated group (76.11 +/- 3.46%, p=0.03). We also observed that CD4(+) and CD8(+) T cells in spleens and in damaged skin, as well as the percentage of neutrophils in the burned area, were modulated by MSC treatment. Plasma cytokine (TGF-beta, IL-10, IL-6, and CINC-1) levels were also altered in the MSC-treated rats, when compared to controls. Number of injected GFP(+) MSCs progressively decreased over time, and 60 days after injection, few MSCs were still detected in the skin of treated animals. This study demonstrates the therapeutic effectiveness of intradermal application of MSCs in a rat model of deep burns, providing basis for future regenerative therapies in patients suffering from deep burn injuries. (AU)