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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Salmonella enterica Typhimurium fljBA operon stability: implications regarding the origin of Salmonella enterica I 4,[5], 12:i:-

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Author(s):
Tomiyama, M. P. O. [1] ; Werle, C. H. [1] ; Milanez, G. P. [1] ; Nobrega, D. B. [1] ; Pereira, J. P. [1] ; Calarga, A. P. [1] ; Flores, F. [2] ; Brocchi, M. [1]
Total Authors: 8
Affiliation:
[1] Univ Estadual Campinas, Inst Biol, Dept Genet Evolucao & Bioagentes, Campinas, SP - Brazil
[2] Fac Murialdo, Fac Agr Engn, Caxias Do Sul, RS - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Genetics and Molecular Research; v. 14, n. 4, p. 19057-19065, 2015.
Web of Science Citations: 0
Abstract

Salmonella enterica subsp enterica serovar 4,5, 12:i:- has been responsible for many recent Salmonella outbreaks worldwide. Several studies indicate that this serovar originated from S. enterica subsp enterica serovar Typhimurium, by the loss of the flagellar phase II gene (fljB) and adjacent sequences. However, at least two different clones of S. enterica 4,5, 12:i:- exist that differs in the molecular events responsible for fljB deletion. The aim of this study was to test the stability of the fljBA operon responsible for the flagellar phase variation under different growth conditions in order to verify if its deletion is a frequent event that could explain the origin and dissemination of this serovar. In fact, coding sequences for transposons are present near this operon and in some strains, such as S. enterica Typhimurium LT2, the Fels-2 prophage gene is inserted near this operon. The presence of mobile DNA could confer instability to this region. In order to examine this, the cat (chloramphenicol acetyltransferase) gene was inserted adjacent to the fljBA operon so that deletions involving this genomic region could be identified. After growing S. enterica chloramphenicol-resistant strains under different conditions, more than 104 colonies were tested for the loss of chloramphenicol resistance. However, none of the colonies were sensitive to chloramphenicol. These data suggest that the origin of S. enterica serovar 4,5, 12:i:- from Typhimurium by fljBA deletion is not a frequent event. The origin and dissemination of 4,5, 12:i:- raise several questions about the role of flagellar phase variation in virulence. (AU)

FAPESP's process: 09/15956-7 - Salmonella enterica: genetic variability and origin of the serotype 4,[5],12:i:- in Brazil and development of attenuated mutants of S. enterica Enteritidis
Grantee:Marcelo Brocchi
Support type: Regular Research Grants
FAPESP's process: 14/11280-7 - Construction and characterization of double mutant of Salmonella Typhimurium for DNA binding proteins
Grantee:Juliana Pastorello Pereira
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 13/11880-1 - Antimicrobial resistance profile in Salmonella enterica isolated from poultry and detection of invA gene in serovars Senftenberg and Saint Paul
Grantee:Aline Parolin Calarga
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 12/10608-3 - Phenotypic and genotypic profile of antimicrobial resistance in Klebsiella pneumoniae, Salmonella enterica and coagulase-negative Staphylococcus
Grantee:Diego Borin Nóbrega
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 12/05382-6 - Virulence evaluation and Differential gene expression in vivo of Salmonella enterica mutants for nucleoid associated proteins
Grantee:Guilherme Paier Milanez
Support type: Scholarships in Brazil - Doctorate