At-Home Bleaching: Color Alteration, Hydrogen Peroxide Diffusion and Cytotoxicity

Author(s)

Leticia Cunha Amaral Gonzaga de Almeida ^[1] ; Diana Gabriela Soares ^[2] ; Fernanda Almeida Azevedo ^[3] ; Marjorie de Oliveira Gallinari ^[4] ; Carlos Alberto de Souza Costa ^[5] ; Paulo Henrique dos Santos ^[6] ; André Luiz Fraga Briso ^[7]

Affiliation

^[1] Univ Estadual Paulista. Araçatuba School of Dentistry. Department of Restorative Dentistry - Brasil

^[2] Univ Estadual Paulista. Araraquara School of Dentistry. Department of Physiology and Pathology - Brasil

^[3] Univ Estadual Paulista. Araçatuba School of Dentistry. Department of Restorative Dentistry - Brasil

^[4] Univ Estadual Paulista. Araçatuba School of Dentistry. Department of Restorative Dentistry - Brasil

^[5] Univ Estadual Paulista. Araraquara School of Dentistry. Department of Physiology and Pathology - Brasil

^[6] Univ Estadual Paulista. Araçatuba School of Dentistry. Department of Dental Materials and Prosthodontics - Brasil

^[7] Univ Estadual Paulista. Araçatuba School of Dentistry. Department of Restorative Dentistry - Brasil

• Document type: Journal article
• Source: Brazilian Dental Journal; v. 26, n. 4, p. 378-383, 2015-08-00.

Abstract

<p>This study evaluated the color change, cytotoxicity and hydrogen peroxide (HP) diffusion caused by different home bleaching protocols: 10% carbamide peroxide (CP) for 3 or 1.5 h, 6% hydrogen peroxide for 1.5 h or 45 min. To quantify the peroxide penetration, disks of bovine teeth were placed in artificial pulp chambers (APCs) containing acetate buffer, which was collected for evaluation in a spectrophotometer. For analysis of cytotoxicity, specimens were adapted in APCs containing culture medium, which subsequently was applied on MDPC-23 odontoblast-like cells for 1 h. Cellular metabolism was evaluated by methyl tetrazolium (MTT) assay and the color change of the specimens was analyzed using the CIE L * a * b * system. The data were submitted to ANOVA and Fisher test (α=5%). The treatment with 10% CP for 3 h was the most effective, and 6% HP for 45 min produced the lowest color change. The groups 10% CP for 1.5 h and 6% HP for 45 min had the lowest trans-enamel dentinal HP penetration, and the 6% HP for 1.5 h had the highest. None of the protocols affected cellular metabolism and morphology. In conclusion, reduced peroxide exposure time reduced the bleaching result; higher HP diffusion did not mean higher effectiveness.</p> (AU)

• FAPESP's process: 10/10378-2 - Color alteration and trans-enamel,and trans-dentinal H2O2 penetration and cytotoxicity in bleached teeth
• Grantee: Letícia Cunha Amaral Gonzaga de Almeida
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 10/17637-3 - Color alteration and trans-enamel, and trans-dentinal H2O2 penetration and cytotoxicity in bleached teeth
• Grantee: André Luiz Fraga Briso
• Support Opportunities: Regular Research Grants