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Color alteration and trans-enamel,and trans-dentinal H2O2 penetration and cytotoxicity in bleached teeth

Grant number: 10/10378-2
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): July 01, 2011
Effective date (End): June 30, 2013
Field of knowledge:Health Sciences - Dentistry - Dental Materials
Principal Investigator:André Luiz Fraga Briso
Grantee:Letícia Cunha Amaral Gonzaga de Almeida
Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil


The present study aims to evaluate the color alteration, trans-enameland trans-dentinal cytotoxicity and diffusion of hydrogen peroxide (HP) on bovine enamel / dentin specimens, which are divided into 9 groups. The specimens of groups 2-9 will be pigmented with hemolysate blood. Groups 1 (no pigmentation) and 2 will be control groups, without blwaching treatment. The Groups 3 and 4 will receive 10% carbamide peroxide (CP), for 90 or 180 minutes, respectively; the Groups 5 and 6 will receive 6% HP for 90 or 45 minutes,respectively, Group 7 will receive 20% HP, for 50 minutes. The groups 3-6 will receive treatment for 21 days. The groups 8 and 9 will receive 35% HP, in Group 8 the bleaching gel will beapplied and exchanged two times in every 15 minutes and group 9 bleachimg gel will be applied only once,both totaling 45 minutes. Groups 7, 8 and 9 will receive bleaching three sessions, one for week. To quantify the penetration of the peroxide, the specimens will be positioned in artificial pulp chamber (APB) containing acetate buffer solution, which will be collected for evaluation of optical density in a spectrophotometer. The analysis of the color change will be performed by reflection spectrophotometry. The readings will be performed 7 days after each in-office bleaching session or after each week of at- home bleaching and 7 days after the end of treatment. For cytotoxicity analysis, the specimens will be adapted in CPAs, and extracts (products of bleaching + diffusion culture medium) will be applied on odontoblast like cells MDPC- 23 culture for 1 hour. The viability and cell morphology will be evaluated by the MTT assay and SEM, respectively. Results will be subjected to appropriate statistical analysis.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LETICIA CUNHA AMARAL GONZAGA DE ALMEIDA; DIANA GABRIELA SOARES; FERNANDA ALMEIDA AZEVEDO; MARJORIE DE OLIVEIRA GALLINARI; CARLOS ALBERTO DE SOUZA COSTA; PAULO HENRIQUE DOS SANTOS; ANDRÉ LUIZ FRAGA BRISO. At-Home Bleaching: Color Alteration, Hydrogen Peroxide Diffusion and Cytotoxicity. Brazilian Dental Journal, v. 26, n. 4, p. 378-383, . (10/17637-3, 10/10378-2)
AMARAL GONZAGA DE ALMEIDA, LETICIA CUNHA; SOARES, DIANA GABRIELA; GALLINARI, MARJORIE OLIVEIRA; DE SOUZA COSTA, CARLOS ALBERTO; DOS SANTOS, PAULO HENRIQUE; FRAGA BRISO, ANDRE LUIZ. Color alteration, hydrogen peroxide diffusion, and cytotoxicity caused by in-office bleaching protocols. CLINICAL ORAL INVESTIGATIONS, v. 19, n. 3, p. 673-680, . (10/17637-3, 10/10378-2)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ALMEIDA, Letícia Cunha Amaral Gonzaga de. Avaliação da alteração de cor, difusão de peróxido de hidrogênio e citotoxicidade trans-amelodentinária causadas por diferentes técnicas de clareamento dental. 2013. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba Araçatuba.

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