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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Transcription profile of Trichophyton rubrum conidia grown on keratin reveals the induction of an adhesin-like protein gene with a tandem repeat pattern

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Author(s):
Bitencourt, Tamires Aparecida [1] ; Macedo, Claudia [2] ; Franco, Matheus Eloy [1, 3] ; Assis, Amanda Freire [2] ; Komoto, Tatiana Takahasi [1] ; Stehling, Eliana Guedes [4] ; Beleboni, Rene Oliveira [1] ; Malavazi, Iran [5] ; Marins, Mozart [1] ; Fachin, Ana Lucia [1]
Total Authors: 10
Affiliation:
[1] Univ Ribeirao Preto, Unidade Biotecnol, Ave Costabile Romano 2201, BR-14096900 Ribeirao Preto, SP - Brazil
[2] Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto - Brazil
[3] Inst Fed Sul Minas, Campus Machado, Machado - Brazil
[4] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Ribeirao Preto - Brazil
[5] Univ Fed Sao Carlos, CCBS, Dept Genet & Evolucao, BR-13560 Sao Carlos, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: BMC Genomics; v. 17, MAR 18 2016.
Web of Science Citations: 8
Abstract

Background: Trichophyton rubrum is a cosmopolitan filamentous fungus that can infect human keratinized tissue (skin, nails and, rarely, hair) and is the major agent of all chronic and recurrent dermatophytoses. The dermatophyte infection process is initiated through the release of arthroconidial adhesin, which binds to the host stratum corneum. The conidia then germinate, and fungal hyphae invade keratinized skin structures through the secretion of proteases. Although arthroconidia play a central role in pathogenesis, little is known about the dormancy and germination of T. rubrum conidia and the initiation of infection. The objective of this study was to evaluate the transcriptional gene expression profile of T. rubrum conidia during growth on keratin-or elastin-containing medium, mimicking superficial and deep dermatophytosis, respectively. Results: A transcriptional profiling analysis was conducted using a custom oligonucleotide-based microarray by comparing T. rubrum conidia grown on elastin and keratin substrates. This comparison shows differences according to protein source used, but consisted of a very small set of genes, which could be attributed to the quiescent status of conidia. The modulated genes were related to the dormancy, survival and germination of conidia, including genes involved in the respiratory chain, signal transduction and lipid metabolism. However, an induction of a great number of proteases occurred when T. rubrum was grown in the presence of keratin such as the subtilisin family of proteases (Sub 1 and Sub 3) and leucine aminopeptidase (Lap 1 and Lap 2). Interestingly, keratin also promoted the up-regulation of a gene encoding an adhesin-like protein with a tandem repeat sequence. In silico analysis showed that the protein contains a domain related to adhesin that may play a role in host-pathogen interactions. The expression of this adhesin-like gene was also induced during the co-culture of T. rubrum with a human keratinocyte cell line, confirming its role in fungal-host interactions. Conclusion: These results contribute to the discovery of new targets involved in the adhesion of conidia and the maintenance of conidial dormancy, which are essential for triggering the process of infection and the chronicity of dermatophytosis. (AU)

FAPESP's process: 12/03845-9 - Transcriptome of the dermatophyte Trichophyton rubrum in response to antifungal transchalcona in culture conditions that simulate human skin infection
Grantee:Ana Lucia Fachin Saltoratto
Support type: Regular Research Grants
FAPESP's process: 14/23841-3 - Study of natural compounds with antifungal activity to elucidate the action mechanism
Grantee:Ana Lucia Fachin Saltoratto
Support type: Regular Research Grants
FAPESP's process: 12/02920-7 - Transcriptome of the dermatophyte Trichophyton rubrum in response to antifungal transchalcona in culture conditions that simulate infection
Grantee:Tamires Aparecida Bitencourt
Support type: Scholarships in Brazil - Doctorate