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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The effects of ethanol on the size-exclusion characteristics of type I dentin collagen to adhesive resin monomers

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Author(s):
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Chiba, A. [1] ; Zhou, J. [2] ; Nakajima, M. [1] ; Tan, J. [2] ; Tagami, J. [1] ; Scheffel, D. L. S. [3] ; Hebling, J. [3] ; Agee, K. A. [4] ; Breschi, L. [5, 6] ; Gregoire, G. [7] ; Jang, S. S. [8] ; Tay, F. R. [4] ; Pashley, D. H. [4]
Total Authors: 13
Affiliation:
[1] Tokyo Med & Dent Univ, Dept Cariol & Operat Dent, Tokyo - Japan
[2] Peking Univ, Sch & Hosp Stomatol, Dept Prosthodont, Beijing 100871 - Peoples R China
[3] Univ Estadual Paulista UNESP, Araraquara Dent Sch, Dept Orthodont & Pediat Dent, Sao Paulo - Brazil
[4] Augusta Univ, Dent Coll Georgia, Dept Oral Biol, Augusta, GA 30912 - USA
[5] Univ Bologna, Dept Biomed & Neuromotor Sci DIBINEM, Bologna - Italy
[6] IGM CNR Unit Bologna, Bologna - Italy
[7] Univ Toulouse 3, Fac Odontol, Dept Biomat, F-31062 Toulouse - France
[8] Georgia Inst Technol, Dept Mat Sci Engn, Atlanta, GA 30332 - USA
Total Affiliations: 8
Document type: Journal article
Source: Acta Biomaterialia; v. 33, p. 235-241, MAR 15 2016.
Web of Science Citations: 4
Abstract

During dentin bonding with etch-and-rinse adhesive systems, phosphoric acid etching of mineralized dentin solubilizes the mineral crystallites and replaces them with bound and unbound water. During the infiltration phase of dentin bonding, solvated adhesive resin comonomers are supposed to replace all of the unbound collagen water and polymerize into copolymers. A recently published review suggested that dental monomers are too large to enter and displace water from tightly-packed collagen molecules. Conversely, recent work from the authors' laboratory demonstrated that HEMA and TEGDMA freely equilibrate with water-saturated dentin matrices. However, because adhesive blends are solvated in organic solvents, those solvents may remove enough free water to allow collagen molecules to come close enough to exclude adhesive monomer permeation. The present study analyzed the size-exclusion characteristics of dentin collagen, using a gel permeation-like column chromatography technique, filled with dentin powder instead of Sephadex beads as the stationary phase. The elution volumes of different sized test molecules, including adhesive resin monomers, studied in both water saturated dentin, and again in ethanol-dehydrated dentin powder, showed that adhesive resin monomers can freely diffuse into both hydrated and dehydrated collagen molecules. Under these in vitro conditions, all free and some of the loosely-bound water seems to have been removed by ethanol. These results validate the concept that adhesive resin monomers can permeate tightly-bound water in ethanol-saturated collagen molecules during infiltration by etch-and-rinse adhesives. Statement of Significance It has been reported that collagen molecules in dentin matrices are packed too close together to allow permeation of adhesive monomers between them. Resin infiltration, in this view, would be limited to extrafibrillar spaces. Our work suggests that monomers equilibrate with collagen water in both water and ethanol-saturated dentin matrices. (C) 2016 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 14/18160-7 - Hydrophobic monomers infiltration into demineralized dentin using HEMA wet-bonding. A proof of concept
Grantee:Josimeri Hebling Costa
Support Opportunities: Scholarships abroad - Research
FAPESP's process: 14/17232-4 - Hydrophobic monomers infiltration into demineralized dentin using HEMA wet-bonding: a proof of concept
Grantee:Débora Lopes Salles Scheffel
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor