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Hydrophobic monomers infiltration into demineralized dentin using HEMA wet-bonding: a proof of concept

Grant number: 14/17232-4
Support type:Scholarships abroad - Research Internship - Post-doctor
Effective date (Start): February 01, 2015
Effective date (End): January 31, 2016
Field of knowledge:Health Sciences - Dentistry - Dental Materials
Principal Investigator:Josimeri Hebling Costa
Grantee:Débora Lopes Salles Scheffel
Supervisor abroad: David H. Pashley
Home Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Local de pesquisa : Georgia Regents University, United States  
Associated to the scholarship:13/20585-3 - PROTEOLYTIC ACTIVITY OF PRIMARY AND PERMANENT DENTIN AND EFFECT OF EDC APPLICATION ON MMPS INACTIVATION AND ADHESIVE INTERFACE DEGRADATION. IN VITRO AND IN VIVO STUDIES, BP.PD

Abstract

This project aims to investigate the permeability of mineralized and demineralized dentin against different solvents when used as carriers of hydrophobic monomers and elucidate the HEMA wet-bonding concept. Six hundred (600) sound human third molars will be collected and used for the production of powdered dentin. The dentin powder (25 cm3) will be placed in a glass column (30.0 cm x 1.0 cm) that will be used for size-exclusion chromatography and elution of large (blue dextran and albumin) and small molecules (HEMA, TEGDMA, and BIS-GMA). Initially it will be evaluated using mineralized dentin in the presence of Tris buffer (control), acetone, ethanol and HEMA. Then, the column will be completely demineralized with formic acid 0.1 M (pH 2.3) over three days at 4°C and the permeability of demineralized collagen will be analyzed as described for the mineralized dentin. The monomers will be separated and quantified by HPLC. Finally, the column will be equilibrated with 5, 10, 15 or 20% water, and all tests will be repeated to ascertain the minimum water concentration for monomer permeation.When the demineralized dentin powder in the column is equilibrated with water, the endogenous proteases of dentin (MMPs and cysteine cathepsins) will release telopeptide fragments as collagen degradation products that will be identified by ICTP and CTX assays. Data set of response variables will be evaluated for adherence to the normal curve and homoscedasticity of variance. Compliance with the requirements of normality and homogeneity among groups, will allow the application of parametric tests considering paired data; otherwise the alternative nonparametric tests will be used. All statistical inferences will be considered at 5% significance level. (AU)

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SALLES SCHEFFEL, DEBORA LOPES; CURY, JAIME APARECIDO; ANDALO TENUTA, LIVIA MARIA; SCHEFFEL, REGIS HENKE; PEREZ, CRISTINA; SOARES, DIANA GABRIELA; BASSO, FERNANDA GONCALVES; DE SOUZA COSTA, CARLOS ALBERTO; PASHLEY, DAVID HENRY; HEBLING, JOSIMERI. Proteolytic activity, degradation, and dissolution of primary and permanent teeth. International Journal of Paediatric Dentistry, v. 30, n. 5, p. 650-659, SEP 2020. Web of Science Citations: 0.
SALLES SCHEFFEL, DEBORA LOPES; CURY, JAIME APARECIDO; ANDALO TENUTA, LIVIA MARIA; SCHEFFEL, REGIS HENKE; PEREZ, CRISTINA; SOARES, DIANA GABRIELA; BASSO, FERNANDA GONCALVES; DE SOUZA COSTA, CARLOS ALBERTO; PASHLEY, DAVID HENRY; HEBLING, JOSIMERI. Proteolytic activity, degradation, and dissolution of primary and permanent teeth. International Journal of Paediatric Dentistry, MAR 2020. Web of Science Citations: 1.
ZHOU, JIANFENG; CHIBA, AYAKA; SCHEFFEL, DEBORA L. S.; HEBLING, JOSIMERI; AGEE, KELLI; TAGAMI, JUNJI; TAN, JIANQUO; ABUELENAIN, DALIA; ABU NAWAREG, MANAR; HASSAN, ALI H.; BRESCHI, LORENZO; TAY, FRANKLIN R.; PASHLEY, DAVID H. Cross-linked dry bonding: A new etch-and-rinse technique. Dental Materials, v. 32, n. 9, p. 1124-1132, SEP 2016. Web of Science Citations: 8.
ZHOU, JIANFENG; CHIBA, AYAKA; SCHEFFEL, DEBORA L. S.; HEBLING, JOSIMERI; AGEE, KELLI; NIU, LI-NA; TAY, FRANKLIN R.; PASHLEY, DAVID H. Effects of a Dicalcium and Tetracalcium Phosphate-Based Desensitizer on In Vitro Dentin Permeability. PLoS One, v. 11, n. 6 JUN 30 2016. Web of Science Citations: 1.
CHIBA, A.; ZHOU, J.; NAKAJIMA, M.; TAN, J.; TAGAMI, J.; SCHEFFEL, D. L. S.; HEBLING, J.; AGEE, K. A.; BRESCHI, L.; GREGOIRE, G.; JANG, S. S.; TAY, F. R.; PASHLEY, D. H. The effects of ethanol on the size-exclusion characteristics of type I dentin collagen to adhesive resin monomers. Acta Biomaterialia, v. 33, p. 235-241, MAR 15 2016. Web of Science Citations: 4.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.