Proteolytic activity, degradation, and dissolution of primary and permanent teeth

Author(s)

Salles Scheffel, Debora Lopes ^{[1, 2]} ; Cury, Jaime Aparecido ^[3] ; Andalo Tenuta, Livia Maria ^{[4, 3]} ; Scheffel, Regis Henke ^[5] ; Perez, Cristina ^[6] ; Soares, Diana Gabriela ^{[7, 8]} ; Basso, Fernanda Goncalves ^{[7, 9]} ; de Souza Costa, Carlos Alberto ^[7] ; Pashley, David Henry ^[5] ; Hebling, Josimeri ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Sch Dent, Dept Orthodont & Pediat Dent, Araraquara, SP - Brazil

^[2] State Univ Maringa UEM, Dept Dent, Maringa, Parana - Brazil

^[3] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, UNICAMP, Piracicaba, SP - Brazil

^[4] Univ Michigan, Sch Dent, Dept Cariol Restorat Sci & Endodont, Ann Arbor, MI 48109 - USA

^[5] Augusta Univ, Dent Coll Georgia, Dept Oral Biol, Augusta, GA - USA

^[6] Univ Kentucky, Div Pediat Dent, Lexington, KY - USA

^[7] Sao Paulo State Univ UNESP, Sch Dent, Dept Physiol & Patol, Araraquara, SP - Brazil

^[8] Sao Paulo Univ USP, Bauru Sch Dent, Dept Operat Dent Endodont & Dent Mat, Bauru, SP - Brazil

^[9] Univ Ribeirao Preto Unaerp, Dept Physiol & Pathol, Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: International Journal of Paediatric Dentistry; MAR 2020.
• Web of Science Citations: 1

Abstract

Background Primary and permanent teeth composition may influence dissolution and degradation rates. Aim To compare the dissolution and degradation of primary and permanent teeth. Design Enamel and dentin powders were obtained from primary molars and premolars and incubated within different pH buffers. Calcium and inorganic phosphate release was quantified in the buffers by atomic absorption and light spectrophotometry. A colorimetric assay was used to assess the MMP activity of primary dentin (PrD) and permanent dentin (PeD). Collagen degradation was assessed by dry mass loss, change in elastic modulus (E), and ICTP and CTX release. Data were submitted to ANOVA and Tukey's tests (alpha = 0.05). Results Similar dissolution was found between PrD and PeD after 256 hours. At pH 4.5, enamel released more minerals than dentin whereas at pH 5.5 the inverse result was observed. MMP activity was similar for both substrates. PrD showed higher dry mass loss after 1 week. In general, greater reduction inEwas recorded for PrD. Higher quantities of ICTP and CTX were released from PrD after 1 week. Conclusions Primary and permanent teeth presented similar demineralization rates. Collagen degradation, however, was faster and more substantial for PrD. (AU)

• FAPESP's process: 14/18160-7 - Hydrophobic monomers infiltration into demineralized dentin using HEMA wet-bonding. A proof of concept
• Grantee: Josimeri Hebling Costa
• Support Opportunities: Scholarships abroad - Research
• FAPESP's process: 08/02278-8 - Acid etching time of sound and caries-affected dentin of deciduous and permanent teeth: effect on substrate dissolution and resin-dentin bond strength
• Grantee: Débora Lopes Salles Scheffel
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 09/07924-8 - Acid etching time of sound and caries-affected dentin of decidous and permanent teeth: effect on the substrate solubility and production of the resin-dentin bond
• Grantee: Josimeri Hebling Costa
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/17232-4 - Hydrophobic monomers infiltration into demineralized dentin using HEMA wet-bonding: a proof of concept
• Grantee: Débora Lopes Salles Scheffel
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor