| Full text | |
| Author(s): |
Martins-Pinheiro, Marinalva
[1, 2]
;
Schons-Fonseca, Luciane
[3]
;
da Silva, Josefa B.
[3]
;
Domingos, Renan H.
[3]
;
Santos Momo, Leonardo Hiroyuki
;
Quirino Simoes, Ana Carolina
[4]
;
Ho, Paulo Lee
[3, 5]
;
da Costa, Renata M. A.
[6]
Total Authors: 8
|
| Affiliation: | [1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Escola Politecn, Dept Engn Quim, Sao Paulo, SP - Brazil
[3] Inst Butantan, Ctr Biotecnol, BR-05503900 Sao Paulo - Brazil
[4] Univ Fed ABC, Ctr Engn Modelagem & Ciencias Sociais Aplicadas, BR-09210170 Santo Andre - Brazil
[5] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508000 Sao Paulo - Brazil
[6] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210170 Santo Andre - Brazil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | Molecular Genetics and Genomics; v. 291, n. 2, p. 703-722, APR 2016. |
| Web of Science Citations: | 4 |
| Abstract | |
Leptospirosis is an emerging zoonosis with important economic and public health consequences and is caused by pathogenic leptospires. The genus Leptospira belongs to the order Spirochaetales and comprises saprophytic (L. biflexa), pathogenic (L. interrogans) and host-dependent (L. borgpetersenii) members. Here, we present an in silico search for DNA repair pathways in Leptospira spp. The relevance of such DNA repair pathways was assessed through the identification of mRNA levels of some genes during infection in animal model and after exposition to spleen cells. The search was performed by comparison of available Leptospira spp. genomes in public databases with known DNA repair-related genes. Leptospires exhibit some distinct and unexpected characteristics, for instance the existence of a redundant mechanism for repairing a chemically diverse spectrum of alkylated nucleobases, a new mutS-like gene and a new shorter version of uvrD. Leptospira spp. shares some characteristics from Gram-positive, as the presence of PcrA, two RecQ paralogs and two SSB proteins; the latter is considered a feature shared by naturally competent bacteria. We did not find a significant reduction in the number of DNA repair-related genes in both pathogenic and host-dependent species. Pathogenic leptospires were enriched for genes dedicated to base excision repair and non-homologous end joining. Their evolutionary history reveals a remarkable importance of lateral gene transfer events for the evolution of the genus. Up-regulation of specific DNA repair genes, including components of SOS regulon, during infection in animal model validates the critical role of DNA repair mechanisms for the complex interplay between host/pathogen. (AU) | |
| FAPESP's process: | 09/17123-2 - Identification and molecular characterization of genes related to the oxidative stress in Leptospira interrogans |
| Grantee: | Renata Maria Augusto da Costa |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 10/51365-0 - Contribution to knowledge of Leptospira interrogans serovar Copenhageni using genomic information |
| Grantee: | Paulo Lee Ho |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 13/16451-1 - In vitro evaluation of the contribution of CCL2/MCP-1 and the importance of chemokines in the mechanisms of resistance and susceptibility to infection with Leptospira sp. |
| Grantee: | Josefa Bezerra da Silva |
| Support Opportunities: | Regular Research Grants |