In vitro embryos production after oocytes treatment with forskolin

Author(s):Show less -

Paschoal, Daniela Martins ^[1] ; Dias Maziero, Rosiara Rosaria ^[1] ; Sudano, Mateus Jose ^[1] ; Guastali, Midyan Daroz ^[1] ; Vergara, Luis Eduardo ^[1] ; Crocomo, Leticia Ferrari ^[1] ; de Lima-Neto, Joao Ferreira ^[1] ; Ona Magalhaes, Luis Carlos ^[1] ; Monteiro, Bianca Andriolo ^[1] ; Rascado, Tatiana da Silva ^[1] ; Martins, Jr., Alicio ^[2] ; Verde Leal, Claudia Lima ^[3] ; Landim-Alvarenga, Fernanda da Cruz ^[1]

Affiliation

^[1] Sao Paulo State Univ, UNESP, Sch Vet Med & Anim Sci FMVZ, Dept Anim Reprod & Vet Radiol, Rubiao Jr S-N, BR-18618970 Botucatu, SP - Brazil

^[2] Sao Paulo State Univ, UNESP, Sch Vet Med FMVA, Dept Clin Surg & Anim Reprod, Aracatuba, SP - Brazil

^[3] Univ Sao Paulo, Sch Anim Sci & Food Engn FZEA, Dept Basic Sci, Pirassununga, SP - Brazil

• Document type: Journal article
• Source: ZYGOTE; v. 24, n. 2, p. 161-171, APR 2016.
• Web of Science Citations: 0

Abstract

The inhibition of nuclear maturation allows time for the oocyte to accumulate molecules that are important for embryonic development. Thus, the objective of this work was to evaluate the effect of blocking oocyte meiosis with the addition of forskolin, an efficient inhibitor of nuclear maturation, in in vitro maturation (IVM) medium. Forskolin was added to the IVM medium for 6 h at concentrations of 0.1 mM, 0.05 mM or 0.025 mM, then the oocytes were allowed to mature in drug-free medium for 18 h. The oocytes were assessed for the stage of nuclear maturation, the activity and distribution of mitochondria, oocyte ultrastructure, the number of viable cells and the apoptosis rate. After forskolin treatment, the oocytes were fertilized in vitro and cultured for 7 days. On day 7, the blastocyst rate, the ultrastructure, the number of intact cells and the apoptosis rate of the blastocysts were measured. No differences were observed for the stage of nuclear maturation of the oocyte, the mitochondrial activity and distribution, the blastocyst rate or total number of intact cells. However, a higher rate of apoptosis was observed in the blastocysts produced from oocytes blocked for 6 h with the higher concentration of forskolin (P < 0.05). We conclude that all the experimental groups reached the MII stage after the addition of forskolin and that the highest concentration of forskolin caused cellular degeneration without harming embryo production on the 7th day. (AU)

• FAPESP's process: 11/08926-4 - Effects of forskolin, butyrolactone I and roscovitine in maturation of bovine oocytes and its implications in resistence to cryopreservation
• Grantee: Fernanda da Cruz Landim
• Support Opportunities: Regular Research Grants