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Identification and characterization of metagenomas and isolated bacterial aimed at bioremediation contamination of soil areas

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Erica Mendes Lopes
Total Authors: 1
Document type: Doctoral Thesis
Press: Jaboticabal. 2016-02-29.
Institution: Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal
Defense date:
Advisor: Eliana Gertrudes de Macedo Lemos; Jackson Antônio Marcondes de Souza

The environmental impacts caused by successive extractions of minerals and compounds generated toxic waste of this activity have been the focus of concern for environmentalists in this context there is a great effort generated for the development of efficient methodologies and cost-effective to remove heavy metals from soils and contaminated water. Biological methods appear as an alternative to conventional methods for the ground of the earth's ecosystems have a multitude of natural resources beyond the great diversity of microorganisms interacting. The same is investment source for the isolation of microorganisms and metagenomic analyzes to search for genes involved in important processes for metabolic and ecological balance, since both in isolation, as in interactions, these organisms play an important role as regards the production of enzymes in biorremoção metal and soil contaminants. This study aimed to isolate, characterize and evaluate the biosorption and bioaccumulation of metals by microorganisms originating from Sabara ecosystems soil, a mining region of Minas Gerais disabled. In addition we sought to evaluate the functional metabolic diversity, for a comparison of the microbiota of Sabara and Brumadinho, combined with search of genes that confer resistance to chromium (CRHA), copper (copA, CopB, cueO) and nickel (Nixa). The isolates were classified into genres such as Burkolderia, Serratia, Bacillus, Stenotrophomonas and Artrobacter, which have the potential for bioremediation of metals from soil and water. However, six of the 32 isolates were resistant to metals copper, chromium and nickel, at a concentration of 500 mg L-1. Of these isolates, two of them, the genus Arthrobacter and Burkolderia (CP15 and CR11) had metabolic capacity to internalize biossorver and heavy metals at different stages of development. As metabolic activity, the microbial community of the perimeter of iron quadrangle soils (MG) has high activity in the degradation of important carbon sources in biogeochemical cycles and bioremediation, which explains the large amount of ORFs found in the notes of sequenced metagenome. the yoke ecosystems and forest that had a greater number of sequences identified for all the genes involved in the resistance process is noteworthy, influx and efflux of metals, and they copper resistance genes, multicooper oxidase ATPase (copA, copB, cueO) multicopper ATPase like (copA, CopB), chrome oxidase (chrA), chrome transport (chrB) and nickel oxidase (nixA). The soils of Sabara and Brumadinho region allocated to, iron quadrangle, have functional diversity for degradation carbon sources such as amino acids and phenolic compounds, and functional genes of great importance for metabolic processes of removing heavy metals by microorganisms, and microorganisms with ability to remove and internalize metals coming from the waste mining being an indication for use in the bioremediation of metals in areas affected by mining. (AU)

FAPESP's process: 12/20022-6 - Prospection of genes related to iron oxidation in metagenomic libraries
Grantee:Erica Mendes Lopes
Support Opportunities: Scholarships in Brazil - Doctorate