Posing for a picture: vesicle immobilization in agarose gel

Author(s)

Lira, Rafael B. ^{[1, 2]} ; Steinkuehler, Jan ^[2] ; Knorr, Roland L. ^[2] ; Dimova, Rumiana ^[2] ; Riske, Karin A. ^[1]

Affiliation

^[1] Univ Fed Sao Paulo, Dept Biofis, Sao Paulo - Brazil

^[2] Max Planck Inst Colloids & Interfaces, Dept Theory & Biosyst, Potsdam - Germany

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 6, MAY 3 2016.
• Web of Science Citations: 16

Abstract

Taking a photo typically requires the object of interest to stand still. In science, imaging is potentiated by optical and electron microscopy. However, living and soft matter are not still. Thus, biological preparations for microscopy usually include a fixation step. Similarly, immobilization strategies are required for or substantially facilitate imaging of cells or lipid vesicles, and even more so for acquiring high-quality data via fluorescence-based techniques. Here, we describe a simple yet efficient method to immobilize objects such as lipid vesicles with sizes between 0.1 and 100 mu m using agarose gel. We show that while large and giant unilamellar vesicles (LUVs and GUVs) can be caged in the pockets of the gel meshwork, small molecules, proteins and micelles remain free to diffuse through the gel and interact with membranes as in agarose-free solutions, and complex biochemical reactions involving several proteins can proceed in the gel. At the same time, immobilization in agarose has no adverse effect on the GUV size and stability. By applying techniques such as FRAP and FCS, we show that the lateral diffusion of lipids is not affected by the gel. Finally, our immobilization strategy allows capturing high-resolution 3D images of GUVs. (AU)

• FAPESP's process: 11/22171-6 - Study of biophysical properties of giant unilamellar vesicles as model for intracellular delivery of material: membrane electroporation and fusion
• Grantee: Rafael Bezerra de Lira
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/07246-5 - Electroporation and fusion of electrically charged vesicles
• Grantee: Rafael Bezerra de Lira
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate