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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Diversity of Sarcocystis spp shed by opossums in Brazil inferred with phylogenetic analysis of DNA coding ITS1, cytochrome B, and surface antigens

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Valadas, Samantha Y. O. B. [1] ; da Silva, Juliana I. G. [1] ; Lopes, Estela Gallucci [1] ; Keid, Lara B. [2] ; Zwarg, Ticiana [3] ; de Oliveira, Alice S. [3] ; Sanches, Thais C. [3] ; Joppert, Adriana M. [3] ; Pena, Hilda F. J. [1] ; Oliveira, Tricia M. F. S. [2] ; Ferreira, Helena L. [2] ; Soares, Rodrigo M. [1]
Total Authors: 12
Affiliation:
[1] Univ Sao Paulo, Dept Prevent Vet Med & Anim Hlth, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Dept Vet Med, Sao Paulo, SP - Brazil
[3] Secretariat Environm & Green, Tech Div Vet Med & Management Wildlife, Sao Paulo, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Experimental Parasitology; v. 164, p. 71-78, MAY 2016.
Web of Science Citations: 4
Abstract

Although few species of Sarcocystis are known to use marsupials of the genus Didelphis as definitive host, an extensive diversity of alleles of surface antigen genes (sag2, sag3, and sag4) has been described in samples of didelphid opossums in Brazil. In this work, we studied 25 samples of Sarcocystis derived from gastrointestinal tract of opossums of the genus Didelphis by accessing the variability of sag2, sag3, sag4, gene encoding cytochrome b (cytB) and first internal transcribed spacer (ITS1). Reference samples of Sarcocystis neurona (SN138) and Sarcocystis falcatula (SF1) maintained in cell culture were also analyzed. We found four allele variants of cytB, seven allele variants of ITSI, 10 allele variants of sag2, 13 allele variants of sag3, and 6 allele variants of sag4. None of the sporocyst-derived sequences obtained from Brazilian opossums revealed 100% identity to SN138 at cytB gene, nor to SN138 or SF1 at ITSI locus. In addition, none of the sag alleles were found identical to either SF1 or SN138 homologous sequences, and a high number of new sag allele types were found other than those previously described in Brazil. Out of ten sag2 alleles, four are novel, while eight out of 13 sag3 alleles are novel and one out of six sag4 alleles is novel. Further studies are needed to clarify if such a vast repertoire of allele variants of Sarcocystis is the consequence of re-assortments driven by sexual exchange, in order to form individuals with highly diverse characteristics, such as pathogenicity, host spectrum, among others or if it only represents allele variants of different species with different biological traits. (C) 2016 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 11/11226-4 - Molecular characterization of Sarcocystis spp. isolates from marsupials of the genus Didelphis spp. through the analysis of mitocondrial and apicoplast genes, internal transcribed spacers (ITS-1) and surface antigen genes
Grantee:Rodrigo Martins Soares
Support type: Regular Research Grants
FAPESP's process: 11/10550-2 - Molecular characterization of Sarcocystis spp. isolates from marsupials of the genus Didelphis spp. through the analysis of mitocondrial and apicoplast genes, Internal Transcribed Spacers (ITS-1) and surface antigen genes
Grantee:Samantha Yuri Oshiro Branco Valadas
Support type: Scholarships in Brazil - Doctorate (Direct)