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Detection and molecular characterization of Sarcocystis spp. in Marsupials (Didelphis spp.) from Campo Grande, MS e São Paulo, SP, Brazil

Grant number: 19/08294-0
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): July 01, 2019
Effective date (End): February 28, 2023
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Pathology
Principal Investigator:Rosangela Zacarias Machado
Grantee:Mariele de Santi
Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
Associated scholarship(s):21/06779-6 - Genomic sequencing of Sarcocystis spp. derived from brazilian Didelphis spp., BE.EP.DR

Abstract

Protozoan of the Sarcocystis genus are coccidia worldwide distributed, known parasites of several systems in avian, reptiles, fish and mammals, including humans. Marsupials of Didelphis genus (opossums) are the known definitive hosts for different species of Sarcocystis, shedding sporocysts in their faeces. S. neurona is the parasite most frequently associated to Equine Protozoal Myeloencephalitis (EPM). The differentiation between Sarcocystis species have been studied for years, by means of molecular analysis and phylogenetic studies. During the last years a wide collection of Sarcocystis strains have been isolated in several hosts. Previous studies demonstrated a great diversity of allele variation for genes encoding Surface Antigens (SAGs) in sporocysts of Sarcocystis shed by opossums. Inversely, the number of allele variants for the first Internal Transcribed Spacer (ITS) was low. Therefore, the present study aims to access the phylogenetic relations among Sarcocystis spp. isolated from free-living opossums from Campo Grande, MS and São Paulo, SP, evaluating molecular markers with different evolutionary rates, from the loci encoding for SAGs, cytochrome c oxidase subunit 1 (cox1), and ITS1 of the parasite. This way, it is expected to better understand the real genetic diversity between Sarcocystis, which could be associated with the increase in the risk of disease. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MARIELE DE SANTI; MARCOS ROGÉRIO ANDRÉ; KARIN WERTHER; LUIZ RICARDO GONÇALVES; RODRIGO MARTINS SOARES; HEITOR MIRAGLIA HERRERA; FILIPE MARTINS SANTOS; NAYARA YOSHIE SANO; WILLIAM OLIVEIRA DE ASSIS; ANDREZA CASTRO RUCCO; et al. Diversidade molecular de Sarcocystis spp. em gambás (Didelphis spp.) do Sudeste e Centro-oeste do Brasil. Revista Brasileira de Parasitologia Veterinária, v. 32, n. 1, . (19/08294-0, 19/09915-8)
THIAGO MERIGHI VIEIRA DA SILVA; MARIELE DE SANTI; LUIZ RICARDO GONÇALVES; MÁRCIA MARIZA JUSI MERINO; MARCOS ROGÉRIO ANDRÉ; ROSANGELA ZACARIAS MACHADO. Reatividade contra Sarcocystis neurona e Sarcocystis falcatula-like em equinos das regiões Sudeste e Centro-Oeste do Brasil. Revista Brasileira de Parasitologia Veterinária, v. 32, n. 3, . (19/09915-8, 19/08294-0, 20/06817-2)
DE SANTI, MARIELE; ANDRE, MARCOS ROGERIO; WERTHER, KARIN; GONCALVES, LUIZ RICARDO; SOARES, RODRIGO MARTINS; HERRERA, HEITOR MIRAGLIA; SANTOS, FILIPE MARTINS; SANO, NAYARA YOSHIE; DE ASSIS, WILLIAM OLIVEIRA; RUCCO, ANDREZA CASTRO; et al. Molecular diversity of Sarcocystis spp. in opossums (Didelphis spp.) from Southeastern and Midwestern Brazil. REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA, v. 32, n. 1, p. 13-pg., . (19/08294-0)
DA SILVA, THIAGO MERIGHI VIEIRA; DE SANTI, MARIELE; GONCALVES, LUIZ RICARDO; MERINO, MARCIA MARIZA JUSI; ANDRE, MARCOS ROGERIO; MACHADO, ROSANGELA ZACARIAS. Reactivity against Sarcocystis neurona and Sarcocystis falcatula-like in horses from Southeastern and Midwestern Brazil. REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA, v. 32, n. 3, p. 7-pg., . (19/08294-0, 20/06817-2, 19/09915-8)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SANTI, Mariele de. Molecular characterization of Sarcocystis spp. from opossums (Didelphis spp.) and serological detection of anti-Sarcocystis spp. antibodies in horses from Southeastern and Midwestern Brazil. 2023. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.

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