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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Synergistic antimicrobial effect of photodynamic therapy and ciprofloxacin

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Author(s):
Ronqui, Maria Rita [1] ; Fogaca de Aguiar Coletti, Tatiana Maria Starck [1] ; de Freitas, Laura Marise [1] ; Miranda, Elaine Toscano [1] ; Fontana, Carla Raquel [1]
Total Authors: 5
Affiliation:
[1] Univ Estadual Paulista UNESP, Fac Ciencias Farmaceut, Rodovia Araraquara Jau, Km 01 S-N, Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 158, p. 122-129, MAY 2016.
Web of Science Citations: 18
Abstract

The occurrence of a variety of pathogens resistant to current antibiotics remains the major problem in medical care, especially when bacterial infections are established as biofilms. In this study, we propose the use of photo dynamic therapy (PDT) as a monotherapy and associated with antibiotic as an alternative treatment. The aim of this study was to analyze the effects of PDT mediated by methylene blue (MB) on Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922) in both biofilm and planktonic phases. Several concentrations of MB and light doses were tested. The bactericidal effects of PDT as a monotherapy did not increase with the concentration of photosensitizer, but were light dose-dependent. In addition, bacteria in biofilms were less affected than cells in the planktonic phase. Although not concentration-dependent, the disruption effect of PDT on biofilms was clearly illustrated by scanning electron microscopy (SEM). We also carried out experiments that evaluated the synergistic effect of photodynamic therapy and the antibiotic ciprofloxacin. The best results were obtained after combination treatment of photodynamic therapy followed by ciprofloxacin on biofilms, which increased bacterial reduction on biofilms, resulting in a 5.4 log reduction for S. aureus biofilm and approximately 7 log for E. coli biofilm. (C) 2016 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 12/21203-4 - Photodynamic therapy on microbial infections
Grantee:Carla Raquel Fontana
Support Opportunities: Regular Research Grants