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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Direct Analysis of Amphetamine Stimulants in a Whole Urine Sample by Atmospheric Solids Analysis Probe Tandem Mass Spectrometry

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Author(s):
Crevelin, Eduardo J. [1] ; Salami, Fernanda H. [1] ; Alves, Marcela N. R. [1] ; De Martinis, Bruno S. [1] ; Crotti, Antnio E. M. [1] ; Moraes, Luiz A. B. [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Ribeirao Preto, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY; v. 27, n. 5, p. 944-947, MAY 2016.
Web of Science Citations: 5
Abstract

Amphetamine-type stimulants (ATS) are among illicit stimulant drugs that are most often used worldwide. A major challenge is to develop a fast and efficient methodology involving minimal sample preparation to analyze ATS in biological fluids. In this study, a urine pool solution containing amphetamine, methamphetamine, ephedrine, sibutramine, and fenfluramine at concentrations ranging from 0.5 pg/mL to 100 ng/mL was prepared and analyzed by atmospheric solids analysis probe tandem mass spectrometry (ASAP-MS/MS) and multiple reaction monitoring (MRM). A urine sample and saliva collected from a volunteer contributor (V1) were also analyzed. The limit of detection of the tested compounds ranged between 0.002 and 0.4 ng/mL in urine samples; the signal-to-noise ratio was 5. These results demonstrated that the ASAP-MS/MS methodology is applicable for the fast detection of ATS in urine samples with great sensitivity and specificity, without the need for cleanup, preconcentration, or chromatographic separation. Thus ASAP-MS/MS could potentially be used in clinical and forensic toxicology applications. (AU)

FAPESP's process: 13/50729-7 - DESI-HPTLC-Bioautography: New strategies to rapid Identification of anti-mastotos bioactive metabolites from actinomycetes.
Grantee:Luiz Alberto Beraldo de Moraes
Support Opportunities: BIOTA-FAPESP Program - Regular Research Grants
FAPESP's process: 13/20094-0 - Evaluation of the anti-parasitary and insecticidal activities of benzofuran derivatives, and study of their gas-phase fragmentation reactions using tandem mass spectrometry
Grantee:Antônio Eduardo Miller Crotti
Support Opportunities: Regular Research Grants
FAPESP's process: 12/06931-3 - Analysis of Stimulants Amphetamines in Urine and Hair Using Desorption Electrospray Ionization (DESI), and Solid Phase Microextraction in Capillary (in - tube SPME) Techniques Coupled with Sequential Mass Spectrometry (MS/MS)
Grantee:Fernanda Helena Salami
Support Opportunities: Scholarships in Brazil - Post-Doctoral