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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Intra and interspecific sequence variation in closely related species of Cereus (CACTACEAE)

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Author(s):
Silva, Gislaine A. R. [1] ; Jojima, Cecilia L. [1] ; Moraes, Evandro M. [1] ; Antonelli, Alexandre [2, 3] ; Manfrin, Maura H. [4, 5] ; Franco, Fernando F. [1]
Total Authors: 6
Affiliation:
[1] Univ Fed Sao Carlos, Dept Biol, Rodovia Joao Leme dos Santos Km 110, BR-18052780 Sorocaba - Brazil
[2] Univ Gothenburg, Gothenburg Bot Garden, Carl Skottsbergs Gata 22 A, S-41319 Gothenburg - Sweden
[3] Univ Gothenburg, Dept Biol & Environm Sci, Carl Skottsbergs Gata 22 A, S-41319 Gothenburg - Sweden
[4] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, Ave Bandeirantes 3900, BR-14040900 Ribeirao Preto - Brazil
[5] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ave Bandeirantes 3900, BR-14040900 Ribeirao Preto - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Biochemical Systematics and Ecology; v. 65, p. 137-142, APR 2016.
Web of Science Citations: 7
Abstract

In comparative phylogenetic and population genetic studies, one of the most crucial steps is to select appropriate DNA markers, a decision based primarily on the estimated variation in markers in cross-taxonomic surveys. To assess whether genetic variation at the intraspecific level in one species predicts the variation in another closely related species we used two congeneric species of Cereus (Cactaceae: Cereeae). We screened and characterized eight noncoding plastid regions (trnS-trnG, atpI-atpH, trnT-trnL, psbD-trnT, petL-psbE, 3'rps16-5'trnk, trnG intron, and trnL intron), and one nuclear gene (PhyC) in Cereus fernambucensis and C. hildmannianus. A total of 40 individuals from 15 populations were characterized according to nucleotide diversity, number of haplotypes, and number of potentially informative characters. The results revealed that nucleotide substitutions and indels are the main source of variation, with the largest divergence between species found in trnS-trnG. The trnL intron and petL-psbE showed intraspecific variability in both species. The psbD-trnT, atpI-atpH, trnS-trnG, and trnT-trnL, which are the most variable regions in one species, showed no variation in the other. Finally, the nuclear gene PhyC showed more resolution between Cereus species than within species. We thus found considerable heterogeneity among widely used plastid markers, even between closely related species, and suggest the use of PhyC as a marker for phylogenetic inference in these species. These results reinforce the need of screening as a preliminary step to conduct phylogeographic or phylogenetic studies in face of unpredictable sequence variation of molecular markers in plants. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 10/19557-7 - Population genetics and phylogeography of the Cereus fernambucensis species (family Cactaceae; tribe Cereeae)
Grantee:Fernando de Faria Franco
Support Opportunities: Regular Research Grants