| Full text | |
| Author(s): |
da Silva, Inara Bastos
[1]
;
de Andrade Batista, Tatiana Pimental
[2]
;
Martines, Roosecelis Brasil
[3]
;
Kanamura, Cristina Takami
[3]
;
Ribeiro Ferreira, Isabelle Martins
[1, 4]
;
Vidal, Jose Ernesto
[2, 5]
;
Pereira-Chioccola, Vera Lucia
[1]
Total Authors: 7
|
| Affiliation: | [1] Ctr Parasitol & Micol, Lab Biol Mol Parasitas & Fungos, Sao Paulo - Brazil
[2] Inst Infectol Emilio Ribas, Sao Paulo - Brazil
[3] Adolfo Lutz Inst, Ctr Patol, BR-01246902 Sao Paulo, SP - Brazil
[4] Supervisao Vigilancia Saude Santo Amaro Cidade Ad, Prefeitura Municipal Sao - Brazil
[5] Univ Sao Paulo, Fac Med, Dept Molestias Infecciosas & Parasitarias, Hosp Clin, BR-05508 Sao Paulo - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Experimental Parasitology; v. 165, p. 16-21, JUN 2016. |
| Web of Science Citations: | 7 |
| Abstract | |
This study investigated the genetic features of Toxoplasma gondii isolated directly in autopsies of HIV-infected patients who died with severe disseminated toxoplasmosis. This retrospective analysis was conducted in a cohort of 15 HIV-infected patients with clinical and laboratory data. They had previous cerebral toxoplasmosis at least 6 months before the disseminated toxoplasmosis episode. The hypothesis was that they were infected with highly virulent parasites due to the condition in which they died. T. gondii genotyping was done directly in DNA extracted from 30 autopsy brain and lung samples (2 per patient) and mutilocus PCR-RFLP genotyping was done using 12 molecular markers. The 30 clinical samples were genotyped successfully in 8 or more loci and six suggestive genotypes were identified. One of them was Toxo DB \#11, previously identified in different domestic animals and virulent in experimental animals. The other five suggestive genotypes identified in 14 patients were not described. TgHuDis1 was the most frequent and was determined in 8 patients. TgHuDis3 and TgHuDis5 were identified in two patients each. TgHuDis2 and TgHuDis4 have been identified in one patient each. These suggestive genotypes could be considered as virulent, since they caused severe tissue damage and had similar characteristics as Toxo \# DB 11. (C) 2016 Elsevier Inc. All rights reserved. (AU) | |
| FAPESP's process: | 14/09496-1 - New diagnostic strategies employed in symptomatic toxoplasmosis |
| Grantee: | Vera Lúcia Pereira Chioccola |
| Support Opportunities: | Regular Research Grants |