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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Rapid turbidimetric assay for quantification of fusidic acid in a dermatological cream

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Author(s):
Curbete, Mariane Machado [1] ; Nunes Salgado, Herida Regina [1]
Total Authors: 2
Affiliation:
[1] UNESP Univ Estadual Paulista, Sch Pharmaceut Sci, Dept Pharmaceut, Campus Araraquara, Rodovia Araraquara Jau, Km 1, BR-14800903 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Talanta; v. 153, p. 51-56, JUN 1 2016.
Web of Science Citations: 5
Abstract

Fusidic acid is an antibiotic steroid widely used for the treatment of serious infections caused by methicillin-resistant Staphylococcus aureus (MRSA) strains. Microbiological methods are indispensable to determine the mean percentage of antimicrobial in medicaments during manufacturing and quality control processes. The aim of this study was to develop and validate a microbiological method for the quantification of fusidic acid in dermatological cream by turbidimetry, using Staphylococcus epidermidis (ATCC 12228) and casoy broth as the culture medium. The validation parameters were in accordance with ICH specifications and demonstrated accuracy, precision, selectivity, and robustness, with linear ranges from 0.25 to 2.25 mu g mL(-1). This method is an alternative to the diffusion agar assay currently employed to quantify fusidic acid in dermatological cream, since it is sensitive; fast, and more economical. (C) 2016 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 13/02861-3 - Chemical-pharmaceutical analysis of fusidic acid in cream formulations
Grantee:Mariane Machado Curbete
Support type: Scholarships in Brazil - Master