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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Unraveling the Sex Chromosome Heteromorphism of the Paradoxical Frog Pseudis tocantins

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Author(s):
Gatto, Kaleb Pretto [1] ; Busin, Carmen Silvia [2] ; Lourenco, Luciana Bolsoni [1]
Total Authors: 3
Affiliation:
[1] Univ Estadual Campinas, Inst Biol, Dept Biol Estrutural & Func, BR-13083863 Sao Paulo - Brazil
[2] Univ Passo Fundo, Inst Ciencias Biol, Lab Citogenet, BR-99001970 Passo Fundo, RS - Brazil
Total Affiliations: 2
Document type: Journal article
Source: PLoS One; v. 11, n. 5 MAY 23 2016.
Web of Science Citations: 8
Abstract

The paradoxical frog Pseudis tocantins is the only species in the Hylidae family with known heteromorphic Z and W sex chromosomes. The Z chromosome is metacentric and presents an interstitial nucleolar organizer region (NOR) on the long arm that is adjacent to a pericentromeric heterochromatic band. In contrast, the submetacentric W chromosome carries a pericentromeric NOR on the long arm, which is adjacent to a clearly evident heterochromatic band that is larger than the band found on the Z chromosome and justify the size difference observed between these chromosomes. Here, we provide evidence that the non-centromeric heterochromatic bands in Zq and Wq differ not only in size and location but also in composition, based on comparative genomic hybridization (CGH) and an analysis of the anuran PcP190 satellite DNA. The finding of PcP190 sequences in P. tocantins extends the presence of this satellite DNA, which was previously detected among Leptodactylidae and Hylodidae, suggesting that this family of repetitive DNA is even older than it was formerly considered. Seven groups of PcP190 sequences were recognized in the genome of P. tocantins. PcP190 probes mapped to the heterochromatic band in Wq, and a Southern blot analysis indicated the accumulation of PcP190 in the female genome of P. tocantins, which suggests the involvement of this satellite DNA in the evolution of the sex chromosomes of this species. (AU)

FAPESP's process: 11/05416-5 - Analysis of the sex chromosomes of Pseudis tocantins (Anura, Hylidae)
Grantee:Kaleb Pretto Gatto
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 14/23542-6 - Comparative molecular cytogenetics of anurans of the genus Pseudis with emphasis on the study of sex chromosomes
Grantee:Luciana Bolsoni Lourenço
Support Opportunities: Regular Research Grants